TY - JOUR
T1 - Long-chain fatty alcohol quantitation in subfemtomole amounts by gas chromatography-negative ion chemical ionization mass spectrometry. Application to long-chain acyl coenzyme A measurement
AU - Wolf, Bryan A.
AU - Conrad-Kessel, Wendy
AU - Turk, John
N1 - Funding Information:
These studies were supported by grants from the U.S. Public Health Service (Grants ROl-DK-34388, K04-DK-01553), from the Council for Tobacco Research
PY - 1990
Y1 - 1990
N2 - We describe a simple and sensitive method to identify and quantitate long-chain fatty alcohols. Long-chain fatty alcohols were converted to their pentafluorobenzoyl derivative and analyzed by gas chromatography (GC)-mass spectrometry in the negative ion chemical ionization (NICI) mode with selected ion monitoring. GC resolution was obtained for myristyl, palmityl, heptadecyl, stearyl, oleyl, linoleyl and arachidonyl alcohols. As little as 0.4 fmol of fatty alcohol can be detected, which represents a six order-of-magnitude increase in sensitivity over previously described methods. This assay can be used to measure femtomolar amounts of long-chain acyl coenzyme A thioesters after reduction to the corresponding fatty alcohols with sodium borohydride. Other potential applications of this assay include identification and quantitation of long-chain fatty alcohol production by microorganisms.
AB - We describe a simple and sensitive method to identify and quantitate long-chain fatty alcohols. Long-chain fatty alcohols were converted to their pentafluorobenzoyl derivative and analyzed by gas chromatography (GC)-mass spectrometry in the negative ion chemical ionization (NICI) mode with selected ion monitoring. GC resolution was obtained for myristyl, palmityl, heptadecyl, stearyl, oleyl, linoleyl and arachidonyl alcohols. As little as 0.4 fmol of fatty alcohol can be detected, which represents a six order-of-magnitude increase in sensitivity over previously described methods. This assay can be used to measure femtomolar amounts of long-chain acyl coenzyme A thioesters after reduction to the corresponding fatty alcohols with sodium borohydride. Other potential applications of this assay include identification and quantitation of long-chain fatty alcohol production by microorganisms.
UR - http://www.scopus.com/inward/record.url?scp=0025362437&partnerID=8YFLogxK
U2 - 10.1016/S0021-9673(01)93090-0
DO - 10.1016/S0021-9673(01)93090-0
M3 - Article
C2 - 2211898
AN - SCOPUS:0025362437
SN - 0021-9673
VL - 509
SP - 325
EP - 332
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -