TY - JOUR
T1 - Location, allocation, relocation
T2 - isolating adult tissue stem cells in three dimensions
AU - Huh, Won Jae
AU - Pan, Xiaoou O.
AU - Mysorekar, Indira U.
AU - Mills, Jason C.
N1 - Funding Information:
We thank the Washington University Digestive Diseases Research Core Center (DDRCC) Murine Models Core, supported by grant #P30 DK52574. JCM is supported by NIH career development award K08 DK066062 and an award from the Diabetes Research and Training Center (DRTC; 5 P60 DK20579).
PY - 2006/10
Y1 - 2006/10
N2 - The literature on isolation of adult tissue stem cells is vast and disparate. To better organize the field, we redefine 'isolation', re-expressing it as the sum of three component vectors: location, allocation and relocation. Location is the isolation of stem cells in situ by anatomical features. Allocation is physical isolation by cell sorting. Relocation is isolation of the functional properties of a stem cell to regenerate its normal progeny when relocated to a new environment. Techniques for the allocation and relocation of stem cells from certain tissues (e.g. hematopoietic) are currently better defined than their location, whereas those of other tissues (e.g. mammary glands) have had recent advances along all three vectors. Yet another group (e.g. gastric glands), have stem cells with well characterized location, emerging techniques for allocation but still rudimentary techniques for relocation.
AB - The literature on isolation of adult tissue stem cells is vast and disparate. To better organize the field, we redefine 'isolation', re-expressing it as the sum of three component vectors: location, allocation and relocation. Location is the isolation of stem cells in situ by anatomical features. Allocation is physical isolation by cell sorting. Relocation is isolation of the functional properties of a stem cell to regenerate its normal progeny when relocated to a new environment. Techniques for the allocation and relocation of stem cells from certain tissues (e.g. hematopoietic) are currently better defined than their location, whereas those of other tissues (e.g. mammary glands) have had recent advances along all three vectors. Yet another group (e.g. gastric glands), have stem cells with well characterized location, emerging techniques for allocation but still rudimentary techniques for relocation.
UR - https://www.scopus.com/pages/publications/33748961615
U2 - 10.1016/j.copbio.2006.07.002
DO - 10.1016/j.copbio.2006.07.002
M3 - Review article
C2 - 16889955
AN - SCOPUS:33748961615
SN - 0958-1669
VL - 17
SP - 511
EP - 517
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
IS - 5
ER -