Localizing and tracking single emitters in three dimensions using a Double-Helix Point Spread Function

Michael A. Thompson; Matthew D. Lew; Majid Badieirostami; W. E. Moerner

doi:10.1364/cleo.2010.cthcc3

Localizing and tracking single emitters in three dimensions using a Double-Helix Point Spread Function

Michael A. Thompson, Matthew D. Lew, Majid Badieirostami, W. E. Moerner

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Single fluorescent emitters can be localized and tracked in 3D with less than 1000 detected photons by using a DH-PSF. The tracking of quantum dots both in solution and inside a living cell is demonstrated

Original language	English
Title of host publication	Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference
Subtitle of host publication	2010 Laser Science to Photonic Applications, CLEO/QELS 2010
Publisher	Association for Computing Machinery
ISBN (Print)	9781557528902
DOIs	https://doi.org/10.1364/cleo.2010.cthcc3
State	Published - 2010

Publication series

Name	Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010

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10.1364/cleo.2010.cthcc3

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Thompson, M. A., Lew, M. D., Badieirostami, M., & Moerner, W. E. (2010). Localizing and tracking single emitters in three dimensions using a Double-Helix Point Spread Function. In Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010 Article 5499680 (Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010). Association for Computing Machinery. https://doi.org/10.1364/cleo.2010.cthcc3

Thompson, Michael A. ; Lew, Matthew D. ; Badieirostami, Majid et al. / Localizing and tracking single emitters in three dimensions using a Double-Helix Point Spread Function. Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010. Association for Computing Machinery, 2010. (Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010).

@inproceedings{832dfdbddaeb47ee98d87a5af465812b,

title = "Localizing and tracking single emitters in three dimensions using a Double-Helix Point Spread Function",

abstract = "Single fluorescent emitters can be localized and tracked in 3D with less than 1000 detected photons by using a DH-PSF. The tracking of quantum dots both in solution and inside a living cell is demonstrated",

author = "Thompson, {Michael A.} and Lew, {Matthew D.} and Majid Badieirostami and Moerner, {W. E.}",

year = "2010",

doi = "10.1364/cleo.2010.cthcc3",

language = "English",

isbn = "9781557528902",

series = "Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010",

publisher = "Association for Computing Machinery",

booktitle = "Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference",

}

Thompson, MA, Lew, MD, Badieirostami, M & Moerner, WE 2010, Localizing and tracking single emitters in three dimensions using a Double-Helix Point Spread Function. in Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010., 5499680, Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010, Association for Computing Machinery. https://doi.org/10.1364/cleo.2010.cthcc3

Localizing and tracking single emitters in three dimensions using a Double-Helix Point Spread Function. / Thompson, Michael A.; Lew, Matthew D.; Badieirostami, Majid et al.
Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010. Association for Computing Machinery, 2010. 5499680 (Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Localizing and tracking single emitters in three dimensions using a Double-Helix Point Spread Function

AU - Thompson, Michael A.

AU - Lew, Matthew D.

AU - Badieirostami, Majid

AU - Moerner, W. E.

PY - 2010

Y1 - 2010

N2 - Single fluorescent emitters can be localized and tracked in 3D with less than 1000 detected photons by using a DH-PSF. The tracking of quantum dots both in solution and inside a living cell is demonstrated

AB - Single fluorescent emitters can be localized and tracked in 3D with less than 1000 detected photons by using a DH-PSF. The tracking of quantum dots both in solution and inside a living cell is demonstrated

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DO - 10.1364/cleo.2010.cthcc3

M3 - Conference contribution

AN - SCOPUS:77957551469

SN - 9781557528902

T3 - Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010

BT - Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference

PB - Association for Computing Machinery

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Thompson MA, Lew MD, Badieirostami M, Moerner WE. Localizing and tracking single emitters in three dimensions using a Double-Helix Point Spread Function. In Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010. Association for Computing Machinery. 2010. 5499680. (Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010). doi: 10.1364/cleo.2010.cthcc3

Localizing and tracking single emitters in three dimensions using a Double-Helix Point Spread Function

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