Localization precision of three-dimensional superresolution fluorescence imaging using a double-helix point spread function

Matthew Lew; Michael A. Thompson; Majid Badieirostami; W. E. Moerner

doi:10.1364/cosi.2009.ctha4

Localization precision of three-dimensional superresolution fluorescence imaging using a double-helix point spread function

Matthew Lew, Michael A. Thompson, Majid Badieirostami, W. E. Moerner

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We localize a diffraction-limited fluorescent bead to 10-20 nm in three dimensions using a doublehelix point spread function and use this method to track a fluorescently tagged protein in three dimensions within a live cell.

Original language	English
Title of host publication	Computational Optical Sensing and Imaging, COSI 2009
Publisher	Optical Society of America (OSA)
ISBN (Print)	9781557528780
DOIs	https://doi.org/10.1364/cosi.2009.ctha4
State	Published - 2009
Event	Computational Optical Sensing and Imaging, COSI 2009 - San Jose, CA, United States Duration: Oct 13 2009 → Oct 15 2009

Publication series

Name	Optics InfoBase Conference Papers
ISSN (Electronic)	2162-2701

Conference

Conference	Computational Optical Sensing and Imaging, COSI 2009
Country/Territory	United States
City	San Jose, CA
Period	10/13/09 → 10/15/09

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10.1364/cosi.2009.ctha4

Cite this

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title = "Localization precision of three-dimensional superresolution fluorescence imaging using a double-helix point spread function",

abstract = "We localize a diffraction-limited fluorescent bead to 10-20 nm in three dimensions using a doublehelix point spread function and use this method to track a fluorescently tagged protein in three dimensions within a live cell.",

author = "Matthew Lew and Thompson, {Michael A.} and Majid Badieirostami and Moerner, {W. E.}",

year = "2009",

doi = "10.1364/cosi.2009.ctha4",

language = "English",

isbn = "9781557528780",

series = "Optics InfoBase Conference Papers",

publisher = "Optical Society of America (OSA)",

booktitle = "Computational Optical Sensing and Imaging, COSI 2009",

note = "Computational Optical Sensing and Imaging, COSI 2009 ; Conference date: 13-10-2009 Through 15-10-2009",

}

Lew, M, Thompson, MA, Badieirostami, M & Moerner, WE 2009, Localization precision of three-dimensional superresolution fluorescence imaging using a double-helix point spread function. in Computational Optical Sensing and Imaging, COSI 2009. Optics InfoBase Conference Papers, Optical Society of America (OSA), Computational Optical Sensing and Imaging, COSI 2009, San Jose, CA, United States, 10/13/09. https://doi.org/10.1364/cosi.2009.ctha4

Localization precision of three-dimensional superresolution fluorescence imaging using a double-helix point spread function. / Lew, Matthew; Thompson, Michael A.; Badieirostami, Majid et al.
Computational Optical Sensing and Imaging, COSI 2009. Optical Society of America (OSA), 2009. (Optics InfoBase Conference Papers).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Localization precision of three-dimensional superresolution fluorescence imaging using a double-helix point spread function

AU - Lew, Matthew

AU - Thompson, Michael A.

AU - Badieirostami, Majid

AU - Moerner, W. E.

PY - 2009

Y1 - 2009

N2 - We localize a diffraction-limited fluorescent bead to 10-20 nm in three dimensions using a doublehelix point spread function and use this method to track a fluorescently tagged protein in three dimensions within a live cell.

AB - We localize a diffraction-limited fluorescent bead to 10-20 nm in three dimensions using a doublehelix point spread function and use this method to track a fluorescently tagged protein in three dimensions within a live cell.

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U2 - 10.1364/cosi.2009.ctha4

DO - 10.1364/cosi.2009.ctha4

M3 - Conference contribution

AN - SCOPUS:85087605825

SN - 9781557528780

T3 - Optics InfoBase Conference Papers

BT - Computational Optical Sensing and Imaging, COSI 2009

PB - Optical Society of America (OSA)

T2 - Computational Optical Sensing and Imaging, COSI 2009

Y2 - 13 October 2009 through 15 October 2009

ER -

Localization precision of three-dimensional superresolution fluorescence imaging using a double-helix point spread function

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