TY - JOUR
T1 - Localization of FGF receptor mRNA in the adult rat central nervous system by in situ hybridization
AU - Wanaka, Akio
AU - Johnson, Eugene M.
AU - Milbrand, Jeffrey
N1 - Funding Information:
We thank Ms. Jenny Colombo and Ms. Patricia A. Osborne for their technical assistance, Ms. P. A. Osborne for her skillful illustration, Mr. Mark A. Watson for helpful comments on the manuscript, and Dr. Andrew P. McMahon for his advices on in situ hybridization histochemistry. This work was supported by the Monsanto Co., grant NS 24679 from the National Institutes of Health, grant POI CA49712 from National Cancer Institute, and grant RG 1779B2 from National Multiple Sclerosis Foundation. A. W. is supported by funds provided by an anonymous donor to the Pharmacology Department of Washington University School of Medicine.
PY - 1990/9
Y1 - 1990/9
N2 - Fibroblast growth factor receptor (FGF-R) mRNA expression was examined in the adult rat CNS. Northern blot analysis showed a distinct 4.3 kb transcript in various CNS regions. In situ hybridization revealed widely distributed, but specific, populations of cells that express FGF-R mRNA. The most intense hybridization signals were observed in the hippocampus and in the pontine cholinergic neurons. The limbic system and brainstem nuclei, including motor nuclei, showed robust labeling. Cerebellar granule cells and spinal cord neurons were positive for FGF-R mRNA. The distribution of FGF-R mRNA differed significantly from that of NGF receptor mRNA; particularly, no hybridization signal was detected in basal forebrain cholinergic neurons. These results strongly suggest that FGF or FGF-like molecules may exert effects on specific neuronal populations in the mature CNS.
AB - Fibroblast growth factor receptor (FGF-R) mRNA expression was examined in the adult rat CNS. Northern blot analysis showed a distinct 4.3 kb transcript in various CNS regions. In situ hybridization revealed widely distributed, but specific, populations of cells that express FGF-R mRNA. The most intense hybridization signals were observed in the hippocampus and in the pontine cholinergic neurons. The limbic system and brainstem nuclei, including motor nuclei, showed robust labeling. Cerebellar granule cells and spinal cord neurons were positive for FGF-R mRNA. The distribution of FGF-R mRNA differed significantly from that of NGF receptor mRNA; particularly, no hybridization signal was detected in basal forebrain cholinergic neurons. These results strongly suggest that FGF or FGF-like molecules may exert effects on specific neuronal populations in the mature CNS.
UR - http://www.scopus.com/inward/record.url?scp=0025023987&partnerID=8YFLogxK
U2 - 10.1016/0896-6273(90)90164-B
DO - 10.1016/0896-6273(90)90164-B
M3 - Article
C2 - 2169267
AN - SCOPUS:0025023987
SN - 0896-6273
VL - 5
SP - 267
EP - 281
JO - Neuron
JF - Neuron
IS - 3
ER -