TY - JOUR
T1 - Local processing in neurites of VGluT3-expressing amacrine cells differentially organizes visual information
AU - Hsiang, Jen Chun
AU - Johnson, Keith P.
AU - Madisen, Linda
AU - Zeng, Hongkui
AU - Kerschensteiner, Daniel
N1 - Funding Information:
We thank members of the Kerschensteiner lab and Drs. Josh Morgan and Tim Holy for helpful comments and suggestions throughout this study. This work was supported by the National Institutes of Health (EY023341, EY026978, and EY027411 to DK and the Vision Core Grant EY0268) and by an unrestricted grant from the Research to Prevent Blindness Foundation to the Department of Ophthalmology and Visual Sciences at Washington University.
Publisher Copyright:
© Hsiang et al.
PY - 2017/10/12
Y1 - 2017/10/12
N2 - Neurons receive synaptic inputs on extensive neurite arbors. How information is organized across arbors and how local processing in neurites contributes to circuit function is mostly unknown. Here, we used two-photon Ca2+ imaging to study visual processing in VGluT3-expressing amacrine cells (VG3-ACs) in the mouse retina. Contrast preferences (ON vs. OFF) varied across VG3-AC arbors depending on the laminar position of neurites, with ON responses preferring larger stimuli than OFFresponses. Although arbors of neighboring cells overlap extensively, imaging population activity revealed continuous topographic maps of visual space in the VG3-AC plexus. All VG3-AC neurites responded strongly to object motion, but remained silent during global image motion. Thus, VG3-AC arbors limit vertical and lateral integration of contrast and location information, respectively. We propose that this local processing enables the dense VG3-AC plexus to contribute precise object motion signals to diverse targets without distorting targetspecific contrast preferences and spatial receptive fields.
AB - Neurons receive synaptic inputs on extensive neurite arbors. How information is organized across arbors and how local processing in neurites contributes to circuit function is mostly unknown. Here, we used two-photon Ca2+ imaging to study visual processing in VGluT3-expressing amacrine cells (VG3-ACs) in the mouse retina. Contrast preferences (ON vs. OFF) varied across VG3-AC arbors depending on the laminar position of neurites, with ON responses preferring larger stimuli than OFFresponses. Although arbors of neighboring cells overlap extensively, imaging population activity revealed continuous topographic maps of visual space in the VG3-AC plexus. All VG3-AC neurites responded strongly to object motion, but remained silent during global image motion. Thus, VG3-AC arbors limit vertical and lateral integration of contrast and location information, respectively. We propose that this local processing enables the dense VG3-AC plexus to contribute precise object motion signals to diverse targets without distorting targetspecific contrast preferences and spatial receptive fields.
UR - http://www.scopus.com/inward/record.url?scp=85032960275&partnerID=8YFLogxK
U2 - 10.7554/eLife.31307
DO - 10.7554/eLife.31307
M3 - Article
C2 - 29022876
AN - SCOPUS:85032960275
SN - 2050-084X
VL - 6
JO - eLife
JF - eLife
M1 - e31307
ER -