LKB1 expressed in dendritic cells governs the development and expansion of thymus-derived regulatory T cells

Leonard R. Pelgrom, Thiago A. Patente, Alexey Sergushichev, Ekaterina Esaulova, Frank Otto, Arifa Ozir-Fazalalikhan, Hendrik J.P. van der Zande, Alwin J. van der Ham, Stefan van der Stel, Maxim N. Artyomov, Bart Everts

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Liver Kinase B1 (LKB1) plays a key role in cellular metabolism by controlling AMPK activation. However, its function in dendritic cell (DC) biology has not been addressed. Here, we find that LKB1 functions as a critical brake on DC immunogenicity, and when lost, leads to reduced mitochondrial fitness and increased maturation, migration, and T cell priming of peripheral DCs. Concurrently, loss of LKB1 in DCs enhances their capacity to promote output of regulatory T cells (Tregs) from the thymus, which dominates the outcome of peripheral immune responses, as suggested by increased resistance to asthma and higher susceptibility to cancer in CD11c ΔLKB1 mice. Mechanistically, we find that loss of LKB1 specifically primes thymic CD11b + DCs to facilitate thymic Treg development and expansion, which is independent from AMPK signalling, but dependent on mTOR and enhanced phospholipase C β1-driven CD86 expression. Together, our results identify LKB1 as a critical regulator of DC-driven effector T cell and Treg responses both in the periphery and the thymus.

Original languageEnglish
Pages (from-to)406-419
Number of pages14
JournalCell Research
Volume29
Issue number5
DOIs
StatePublished - May 1 2019

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