Liquid Chromatographic Separation of Enantiomers Using a Chiral β-Cyclodextrin-Bonded Stationary Phase and Conventional Aqueous-Organic Mobile Phases

A chiral stationary phase composed of chemically bonded β-cyclodextrin (β-CD) molecules was used to separate enantiomers of dansylsulfonamlde, β-naphthamlde, or β-naphthyl ester derivatives of amino acids, barbiturates, substituted phenylacetic acids, and dioxolanes. The separations are reasonably rationalized In terms of the Inclusion process between the enantiomers and β-cyclodextrin and consideration of a three-point attachment model. The effects of mobile-phase composition, temperature, and flow rate upon the observed enantiomeric selectivity and resolution were critically assessed. Lastly, a brief prospectus on the usefulness of cyclodextrin chiral stationary phases In high-performance liquid chromatographic enantiomeric separations is presented.