TY - JOUR
T1 - Lipoxygenase metabolites of arachidonic acid do not induce mucus secretion from rabbit intestinal goblet cells in vitro
AU - Phillips, T. E.
AU - Stenson, W. F.
AU - Neutra, M. R.
N1 - Funding Information:
This work was funded by National Institutes of Health ResearchG rants AM21503, DK38.587D, K33165,DK33487 and a Fellowshin Grant from the Cvstic Fibrosis Research Foundation. The excellentt echnical& stance of Teresa L. Phillips and darkrooma ssistanceo f Emily Frisch is gratefully acknowledged.
PY - 1989/7
Y1 - 1989/7
N2 - Lipoxygenase metabolites of arachidonic acid are effective mucus secretagogues in the respiratory tract but their efficacy in the intestinal tract was unknown. Mucosal explants and sheets of epithelial cells isolated from rabbit small and large intestine were exposed to leukotrienes B4, C4, and D4 and monohydroxyeicosatetraenoic acids 5-HETE, 12-HETE, and 15-HETE. Light and electron microscopic inspection of goblet cells in treated tissues failed to detect evidence of recent compound exocytosis of mucin granules or other morphological evidence of secretory activity. These results indicate that lipoxygenase metabolites are not directly responsible for the increased mucus secretion observed in ulcerative colitis.
AB - Lipoxygenase metabolites of arachidonic acid are effective mucus secretagogues in the respiratory tract but their efficacy in the intestinal tract was unknown. Mucosal explants and sheets of epithelial cells isolated from rabbit small and large intestine were exposed to leukotrienes B4, C4, and D4 and monohydroxyeicosatetraenoic acids 5-HETE, 12-HETE, and 15-HETE. Light and electron microscopic inspection of goblet cells in treated tissues failed to detect evidence of recent compound exocytosis of mucin granules or other morphological evidence of secretory activity. These results indicate that lipoxygenase metabolites are not directly responsible for the increased mucus secretion observed in ulcerative colitis.
UR - http://www.scopus.com/inward/record.url?scp=0024383124&partnerID=8YFLogxK
U2 - 10.1016/0952-3278(89)90186-5
DO - 10.1016/0952-3278(89)90186-5
M3 - Article
C2 - 2762368
AN - SCOPUS:0024383124
SN - 0952-3278
VL - 37
SP - 51
EP - 55
JO - Prostaglandins, Leukotrienes and Essential Fatty Acids
JF - Prostaglandins, Leukotrienes and Essential Fatty Acids
IS - 1
ER -