Lipopolysaccharide enhances the transcription of prostaglandin H synthase-2 gene in primary human trophoblasts

E. Y. Anteby, R. D. Johnson, X. Huang, D. K. Dryden, D. M. Nelson, Y. Sadovsky

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

OBJECTIVE: Our purpose was to test the hypothesis that lipopolysaccharide potentiates the transcription of human placental prostaglandin H synthase-2 gene, a rate-limiting enzyme in prostaglandin synthesis. STUDY DESIGN: We transfected normal term cytotrophoblasts with a reporter vector containing either prostaglandin H synthase-1 or prostaglandin H synthase-2 promoters, cloned upstream of a luciferase. We correlated lipopolysaccharide effect on luciferase activity with enzyme expression and prostaglandin E2 production. RESULTS: Lipopolysaccharide (10 μg/ml) enhanced prostaglandin H synthase-2 but not prostaglandin H synthase-1 promoter activity. This effect was abolished by dexamethasone (10-6 mol/L). Platelet-derived growth factor, a known stimulant of prostaglandin H synthase-2 in nonplacental tissue, had no effect on the activity of prostaglandin H synthase-2 promoter. Lipopolysaccharide induced the expression of prostaglandin H synthase-2 messenger ribonucleic acid fivefold to sixfold and caused a 3.5-fold increase in prostaglandin E2 production. CONCLUSION: Lipopolysaccharide enhances the transcription of prostaglandin H synthase-2 gene in human trophoblasts. Enhanced expression of prostaglandin H synthase-2 results in a higher production of prostaglandins, which may alter villous blood flow.

Original languageEnglish
Pages (from-to)469-473
Number of pages5
JournalAmerican journal of obstetrics and gynecology
Volume178
Issue number3
DOIs
StatePublished - 1998

Keywords

  • Lipopolysaccharide
  • Prostaglandin H synthase
  • Prostaglandins
  • Trophoblast

Fingerprint

Dive into the research topics of 'Lipopolysaccharide enhances the transcription of prostaglandin H synthase-2 gene in primary human trophoblasts'. Together they form a unique fingerprint.

Cite this