Lipin-1 Contributes to IL-4 Mediated Macrophage Polarization

Sunitha Chandran, Robert M. Schilke, Cassidy M.R. Blackburn, Aila Yurochko, Rusella Mirza, Rona S. Scott, Brian N. Finck, Matthew D. Woolard

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Macrophage responses contribute to a diverse array of pathologies ranging from infectious disease to sterile inflammation. Polarization of macrophages determines their cellular function within biological processes. Lipin-1 is a phosphatidic acid phosphatase in which its enzymatic activity contributes to macrophage pro-inflammatory responses. Lipin-1 also possesses transcriptional co-regulator activity and whether this activity is required for macrophage polarization is unknown. Using mice that lack only lipin-1 enzymatic activity or both enzymatic and transcriptional coregulator activities from myeloid cells, we investigated the contribution of lipin-1 transcriptional co-regulator function toward macrophage wound healing polarization. Macrophages lacking both lipin-1 activities did not elicit IL-4 mediated gene expression to levels seen in either wild-type or lipin-1 enzymatically deficient macrophages. Furthermore, mice lacking myeloid-associated lipin-1 have impaired full thickness excisional wound healing compared to wild-type mice or mice only lacking lipin-1 enzymatic activity from myeloid cell. Our study provides evidence that lipin-1 transcriptional co-regulatory activity contributes to macrophage polarization and influences wound healing in vivo.

Original languageEnglish
Article number787
JournalFrontiers in immunology
Volume11
DOIs
StatePublished - May 5 2020

Keywords

  • lipin-1
  • macrophage
  • polarization
  • transcriptional coregulator
  • wound healing

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