Lipid metabolism in bone and bone cells II. The in vitro incorporation of [32P]orthophosphate and [14C]serine into lipids of bone and bone cell cultures

Thomas R. Dirksen, Guido V. Marinetti, William A. Peck

    Research output: Contribution to journalArticle

    17 Scopus citations

    Abstract

    1. 1. Calvaria of new-born rats or bone cell cultures were incubated with buffer containing [32P]orthophosphate or [14C]serine to study lipid synthesis in mineralizing tissues. 2. 2. [32P]Orthophosphate was readily incorporated into phospholipids by calvaria and bone cell cultures. As with [14C]glucose or [14C]glycerol (ref. 1), lecithin was more heavily labeled than the other phospholipids. The labeling of sphingomyelin and phosphatidyl serine could not be demonstrated from radioactive phosphate. ATP decreased the incorporation of this isotope into lipids. 3. 3. [14C]Serine gave rise to a variety of radioactive lipids. The synthesis of phosphatidyl serine was enhanced by the addition of both CTP and ATP whereas the labeling of sphingomyelin and phosphatidyl ethanolamine was little affected by these two co-factors.

    Original languageEnglish
    Pages (from-to)80-90
    Number of pages11
    JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
    Volume202
    Issue number1
    DOIs
    StatePublished - Feb 10 1970

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