Lipid membrane editing with peptide cargo linkers in cells and synthetic nanostructures

Hua Pan, Jacob W. Myerson, Olena Ivashyna, Neelesh R. Soman, Jon N. Marsh, Joshua L. Hood, Gregory M. Lanza, Paul H. Schlesinger, Samuel A. Wickline

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Current strategies for deploying synthetic nanocarriers involve the creation of agents that incorporate targeting ligands, imaging agents, and/or therapeutic drugs into particles as an integral part of the formulation process. Here we report the development of an amphipathic peptide linker that enables postformulation editing of payloads without the need for reformulation to achieve multiplexing capability for lipidic nanocarriers. To exemplify the flexibility of this peptide linker strategy, 3 applications were demonstrated: converting nontargeted nanoparticles into targeting vehicles; adding cargo to preformulated targeted nanoparticles for in vivo site-specific delivery; and labeling living cells for in vivo tracking. This strategy is expected to enhance the clinical application of molecular imaging and/or targeted therapeutic agents by offering extended flexibility for multiplexing targeting ligands and/or drug payloads that can be selected after base nanocarrier formulation.

Original languageEnglish
Pages (from-to)2928-2937
Number of pages10
JournalFASEB Journal
Volume24
Issue number8
DOIs
StatePublished - Aug 2010

Keywords

  • Biomarker
  • Liposome
  • Molecular imaging
  • Nanoparticles
  • Targeted drug delivery

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