Lipid-dependent cytotoxicity by the lipase PLRP2 and by PLRP2-positive cytotoxic T lymphocytes (CTLs)

Bryce N. Alves, Kristen Marshall, David L. Tamang, Jeffrey Leong, Doug Redelman, Viki Elliott, Mark E. Lowe, Dorothy Hudig

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

IL-4 induces a lipase, pancreatic lipase related protein 2 (PLRP2), in cytotoxic T lymphocytes (CTLs). Because PLRP2 in semen can mediate lipid-dependent toxicity to sperm, we questioned whether CTL-derived PLRP2 could support similar cytotoxicity toward tumor cells. Recombinant PLRP2 was toxic to P815 tumor cells in 48 h when lipid and another protein, colipase, were present. However, PLRP2-positive CTLs (induced with many lots of IL-4) were unable to mediate lipid-dependent cytotoxicity. Notably, CTLs induced with only one lot of IL-4 had lipid-dependent cytotoxicity. The exceptional lot of IL-4 was effective in multiple experiments at inducing lipid-dependent cytotoxicity. The lipid-dependent cytotoxicity it induced was determined to be perforin-independent. CTLs induced with IL-4 that was unable to induce lipid-dependent cytotoxicity had mRNA for PLRP2 but not mRNA for colipase. Therefore, we added exogenous colipase to the CTL assays but still cytotoxicity was unchanged.We conclude (1) that lipid-dependent cytotoxicity, promoted by the lipase PLRP2 and colipase, will kill tumor cells and (2) that more than PLRP2 alone is required for lipid-dependent cytotoxicity mediated by CTLs.

Original languageEnglish
Pages (from-to)296-308
Number of pages13
JournalCell Biochemistry and Function
Volume27
Issue number5
DOIs
StatePublished - Sep 29 2009
Externally publishedYes

Keywords

  • Cytotoxic T lymphocytes
  • IL-4
  • Linoleic acid
  • Lipase
  • PLRP2

Fingerprint Dive into the research topics of 'Lipid-dependent cytotoxicity by the lipase PLRP2 and by PLRP2-positive cytotoxic T lymphocytes (CTLs)'. Together they form a unique fingerprint.

  • Cite this

    Alves, B. N., Marshall, K., Tamang, D. L., Leong, J., Redelman, D., Elliott, V., Lowe, M. E., & Hudig, D. (2009). Lipid-dependent cytotoxicity by the lipase PLRP2 and by PLRP2-positive cytotoxic T lymphocytes (CTLs). Cell Biochemistry and Function, 27(5), 296-308. https://doi.org/10.1002/cbf.1573