Limits of a localized magnetic resonance spectroscopy assay for ex vivo myocardial triacylglycerol

Robert D. O'Connor; Robert J. Gropler; Linda Peterson; Jean Schaffer; Joseph J.H. Ackerman

doi:10.1016/j.jpba.2007.08.022

Limits of a localized magnetic resonance spectroscopy assay for ex vivo myocardial triacylglycerol

Robert D. O'Connor
, Robert J. Gropler
, Linda Peterson
, Jean Schaffer
, Joseph J.H. Ackerman

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Localized magnetic resonance spectroscopy (LMRS) promises a powerful non-invasive means to determine myocardial triacylglycerol (TAG) in a clinical setting. Here, the linearity, specificity, robustness, precision, and accuracy of an ex vivo mouse-heart LMRS TAG assay are assessed by quantifying the spatial, spectral, and relaxation-induced uncertainties. The protocol, which is based on localization by adiabatic selective refocusing (LASER) using frequency offset corrected inversion (FOCI) pulses, alternating gradient polarity, and simple post-processing, is shown to have good characteristics. The presented protocol has a benchmark, phantom-based, accuracy of 3%, and when applied to ex vivo mouse hearts the accuracy is 6%, making the LMRS assay comparable to the typical destructive bioanalytical assay.

Original language	English
Pages (from-to)	382-389
Number of pages	8
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	45
Issue number	3
DOIs	https://doi.org/10.1016/j.jpba.2007.08.022
State	Published - Nov 5 2007

Keywords

FOCI
LASER
Myocardial TAG
Quantitative localized MRS
T relaxation
Triacylglycerol

Access to Document

10.1016/j.jpba.2007.08.022

Cite this

@article{8678966be95641dda2a9d5e3d33bee1c,

title = "Limits of a localized magnetic resonance spectroscopy assay for ex vivo myocardial triacylglycerol",

abstract = "Localized magnetic resonance spectroscopy (LMRS) promises a powerful non-invasive means to determine myocardial triacylglycerol (TAG) in a clinical setting. Here, the linearity, specificity, robustness, precision, and accuracy of an ex vivo mouse-heart LMRS TAG assay are assessed by quantifying the spatial, spectral, and relaxation-induced uncertainties. The protocol, which is based on localization by adiabatic selective refocusing (LASER) using frequency offset corrected inversion (FOCI) pulses, alternating gradient polarity, and simple post-processing, is shown to have good characteristics. The presented protocol has a benchmark, phantom-based, accuracy of 3\%, and when applied to ex vivo mouse hearts the accuracy is 6\%, making the LMRS assay comparable to the typical destructive bioanalytical assay.",

keywords = "FOCI, LASER, Myocardial TAG, Quantitative localized MRS, T relaxation, Triacylglycerol",

author = "O'Connor, \{Robert D.\} and Gropler, \{Robert J.\} and Linda Peterson and Jean Schaffer and Ackerman, \{Joseph J.H.\}",

note = "Funding Information: The authors are grateful for helpful discussions with Dmitriy A. Yablonskiy and members of the Biomedical MR Laboratory. This work was supported, in part, by NIH grants P20 RR020643 and R24-CA83060 (Small Animal Imaging Resource Program). ",

year = "2007",

month = nov,

day = "5",

doi = "10.1016/j.jpba.2007.08.022",

language = "English",

volume = "45",

pages = "382--389",

journal = "Journal of Pharmaceutical and Biomedical Analysis",

issn = "0731-7085",

number = "3",

}

TY - JOUR

T1 - Limits of a localized magnetic resonance spectroscopy assay for ex vivo myocardial triacylglycerol

AU - O'Connor, Robert D.

AU - Gropler, Robert J.

AU - Peterson, Linda

AU - Schaffer, Jean

AU - Ackerman, Joseph J.H.

N1 - Funding Information: The authors are grateful for helpful discussions with Dmitriy A. Yablonskiy and members of the Biomedical MR Laboratory. This work was supported, in part, by NIH grants P20 RR020643 and R24-CA83060 (Small Animal Imaging Resource Program).

PY - 2007/11/5

Y1 - 2007/11/5

N2 - Localized magnetic resonance spectroscopy (LMRS) promises a powerful non-invasive means to determine myocardial triacylglycerol (TAG) in a clinical setting. Here, the linearity, specificity, robustness, precision, and accuracy of an ex vivo mouse-heart LMRS TAG assay are assessed by quantifying the spatial, spectral, and relaxation-induced uncertainties. The protocol, which is based on localization by adiabatic selective refocusing (LASER) using frequency offset corrected inversion (FOCI) pulses, alternating gradient polarity, and simple post-processing, is shown to have good characteristics. The presented protocol has a benchmark, phantom-based, accuracy of 3%, and when applied to ex vivo mouse hearts the accuracy is 6%, making the LMRS assay comparable to the typical destructive bioanalytical assay.

AB - Localized magnetic resonance spectroscopy (LMRS) promises a powerful non-invasive means to determine myocardial triacylglycerol (TAG) in a clinical setting. Here, the linearity, specificity, robustness, precision, and accuracy of an ex vivo mouse-heart LMRS TAG assay are assessed by quantifying the spatial, spectral, and relaxation-induced uncertainties. The protocol, which is based on localization by adiabatic selective refocusing (LASER) using frequency offset corrected inversion (FOCI) pulses, alternating gradient polarity, and simple post-processing, is shown to have good characteristics. The presented protocol has a benchmark, phantom-based, accuracy of 3%, and when applied to ex vivo mouse hearts the accuracy is 6%, making the LMRS assay comparable to the typical destructive bioanalytical assay.

KW - FOCI

KW - LASER

KW - Myocardial TAG

KW - Quantitative localized MRS

KW - T relaxation

KW - Triacylglycerol

UR - https://www.scopus.com/pages/publications/35348826047

U2 - 10.1016/j.jpba.2007.08.022

DO - 10.1016/j.jpba.2007.08.022

M3 - Article

C2 - 17931816

AN - SCOPUS:35348826047

SN - 0731-7085

VL - 45

SP - 382

EP - 389

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

IS - 3

ER -

Limits of a localized magnetic resonance spectroscopy assay for ex vivo myocardial triacylglycerol

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this