TY - JOUR
T1 - Limits of a localized magnetic resonance spectroscopy assay for ex vivo myocardial triacylglycerol
AU - O'Connor, Robert D.
AU - Gropler, Robert J.
AU - Peterson, Linda
AU - Schaffer, Jean
AU - Ackerman, Joseph J.H.
N1 - Funding Information:
The authors are grateful for helpful discussions with Dmitriy A. Yablonskiy and members of the Biomedical MR Laboratory. This work was supported, in part, by NIH grants P20 RR020643 and R24-CA83060 (Small Animal Imaging Resource Program).
PY - 2007/11/5
Y1 - 2007/11/5
N2 - Localized magnetic resonance spectroscopy (LMRS) promises a powerful non-invasive means to determine myocardial triacylglycerol (TAG) in a clinical setting. Here, the linearity, specificity, robustness, precision, and accuracy of an ex vivo mouse-heart LMRS TAG assay are assessed by quantifying the spatial, spectral, and relaxation-induced uncertainties. The protocol, which is based on localization by adiabatic selective refocusing (LASER) using frequency offset corrected inversion (FOCI) pulses, alternating gradient polarity, and simple post-processing, is shown to have good characteristics. The presented protocol has a benchmark, phantom-based, accuracy of 3%, and when applied to ex vivo mouse hearts the accuracy is 6%, making the LMRS assay comparable to the typical destructive bioanalytical assay.
AB - Localized magnetic resonance spectroscopy (LMRS) promises a powerful non-invasive means to determine myocardial triacylglycerol (TAG) in a clinical setting. Here, the linearity, specificity, robustness, precision, and accuracy of an ex vivo mouse-heart LMRS TAG assay are assessed by quantifying the spatial, spectral, and relaxation-induced uncertainties. The protocol, which is based on localization by adiabatic selective refocusing (LASER) using frequency offset corrected inversion (FOCI) pulses, alternating gradient polarity, and simple post-processing, is shown to have good characteristics. The presented protocol has a benchmark, phantom-based, accuracy of 3%, and when applied to ex vivo mouse hearts the accuracy is 6%, making the LMRS assay comparable to the typical destructive bioanalytical assay.
KW - FOCI
KW - LASER
KW - Myocardial TAG
KW - Quantitative localized MRS
KW - T relaxation
KW - Triacylglycerol
UR - http://www.scopus.com/inward/record.url?scp=35348826047&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2007.08.022
DO - 10.1016/j.jpba.2007.08.022
M3 - Article
C2 - 17931816
AN - SCOPUS:35348826047
SN - 0731-7085
VL - 45
SP - 382
EP - 389
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
IS - 3
ER -