Limits of a localized magnetic resonance spectroscopy assay for ex vivo myocardial triacylglycerol

Robert D. O'Connor, Robert J. Gropler, Linda Peterson, Jean Schaffer, Joseph J.H. Ackerman

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Localized magnetic resonance spectroscopy (LMRS) promises a powerful non-invasive means to determine myocardial triacylglycerol (TAG) in a clinical setting. Here, the linearity, specificity, robustness, precision, and accuracy of an ex vivo mouse-heart LMRS TAG assay are assessed by quantifying the spatial, spectral, and relaxation-induced uncertainties. The protocol, which is based on localization by adiabatic selective refocusing (LASER) using frequency offset corrected inversion (FOCI) pulses, alternating gradient polarity, and simple post-processing, is shown to have good characteristics. The presented protocol has a benchmark, phantom-based, accuracy of 3%, and when applied to ex vivo mouse hearts the accuracy is 6%, making the LMRS assay comparable to the typical destructive bioanalytical assay.

Original languageEnglish
Pages (from-to)382-389
Number of pages8
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume45
Issue number3
DOIs
StatePublished - Nov 5 2007

Keywords

  • FOCI
  • LASER
  • Myocardial TAG
  • Quantitative localized MRS
  • T relaxation
  • Triacylglycerol

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