TY - JOUR
T1 - Library subtraction of in vitro cDNA libraries to identify differentially expressed genes in scrapie infection
AU - Duguid, John R.
AU - Dinauer, Mary C.
N1 - Funding Information:
We would like to thank Brian Seed and Stuart Orkin for helpful comments, and Maryanne Lemaire for technical assistance. This work was supported by grants from the Veterans Administration and the Whitaker Health Sciences Fund.
PY - 1990/5
Y1 - 1990/5
N2 - We have developed a system where double-stranded cDNA can be amplified using a synthetic ollgonucleotide primer and the polymerase chain reaction, generating cDNA libraries in vitro. Using a library subtraction strategy (1), scrapie and control brain in vitro cDNA libraries were used to identify sequences whose expression is modulated In scrapie infection. One of these sequences represents β-2 microglobulin, while the other two have not been previously described. The use of in vitro libraries offers increased speed and efficiency of construction, and their subtraction is more efficient and powerful, compared with the previous system (1).
AB - We have developed a system where double-stranded cDNA can be amplified using a synthetic ollgonucleotide primer and the polymerase chain reaction, generating cDNA libraries in vitro. Using a library subtraction strategy (1), scrapie and control brain in vitro cDNA libraries were used to identify sequences whose expression is modulated In scrapie infection. One of these sequences represents β-2 microglobulin, while the other two have not been previously described. The use of in vitro libraries offers increased speed and efficiency of construction, and their subtraction is more efficient and powerful, compared with the previous system (1).
UR - http://www.scopus.com/inward/record.url?scp=0025319817&partnerID=8YFLogxK
U2 - 10.1093/nar/18.9.2789
DO - 10.1093/nar/18.9.2789
M3 - Article
C2 - 2339063
AN - SCOPUS:0025319817
VL - 18
SP - 2789
EP - 2792
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 9
ER -