TY - JOUR
T1 - Level of cytomegalovirus (CMV) DNA in cerebrospinal fluid of subjects with AIDS and CMV infection of the central nervous system
AU - Arribas, Jose R.
AU - Clifford, David B.
AU - Fichtenbaum, Carl J.
AU - Commins, Deborah L.
AU - Powderly, William G.
AU - Storch, Gregory A.
N1 - Funding Information:
Received 20 June 1994; revised 16 March 1995. Presented in part: 34th Interscience Conference on Antimicrobial Agents and Chemotherapy, Orlando, Florida, 4-7 October (abstract 1151). The study was approved by the Human Studies Committee, Washington University. Grant support: National Institutes of Health (AI-25903, INS-3228). Reprints or correspondence: Dr. Jose R. Arribas, AIDS Clinical Trials Unit, 4511 Forest Park, Suite 304, St. Louis, MO 63108. * Present address: 1800 Alhambra Rd., South Pasadena, CA 91030.
PY - 1995/8
Y1 - 1995/8
N2 - The objective of this study was to correlate cytomegalovirus (CMV) DNA levels in the cerebrospinal fluid (CSF) of subjects with AIDS with clinical and pathologic findings attributable to CMV infection of the central nervous system (CNS). CMV polymerase chain reaction (PCR) was done on serial dilutions of CSF samples from 24 AIDS patients with autopsy-proven CNS disorders. CMV DNA was detected in CSF from 12 of 13 subjects with evidence of CMV infection of the brain or spinal cord but in none of 11 subjects without autopsy evidence of CMV CNS infection. Subjects whose CSF contained >103CMV DNA molecules/8 μL of CSF had severe CMV CNS disease (e.g., ventriculoencephalitis). PCR appears to be more useful than clinical and neuroradiologic findings for documenting CMV infection of the CNS in patients with AIDS. Quantitation of CMV DNA in CSF shows promise for evaluation of the extent of involvement.
AB - The objective of this study was to correlate cytomegalovirus (CMV) DNA levels in the cerebrospinal fluid (CSF) of subjects with AIDS with clinical and pathologic findings attributable to CMV infection of the central nervous system (CNS). CMV polymerase chain reaction (PCR) was done on serial dilutions of CSF samples from 24 AIDS patients with autopsy-proven CNS disorders. CMV DNA was detected in CSF from 12 of 13 subjects with evidence of CMV infection of the brain or spinal cord but in none of 11 subjects without autopsy evidence of CMV CNS infection. Subjects whose CSF contained >103CMV DNA molecules/8 μL of CSF had severe CMV CNS disease (e.g., ventriculoencephalitis). PCR appears to be more useful than clinical and neuroradiologic findings for documenting CMV infection of the CNS in patients with AIDS. Quantitation of CMV DNA in CSF shows promise for evaluation of the extent of involvement.
UR - http://www.scopus.com/inward/record.url?scp=0028984537&partnerID=8YFLogxK
U2 - 10.1093/infdis/172.2.527
DO - 10.1093/infdis/172.2.527
M3 - Article
C2 - 7622897
AN - SCOPUS:0028984537
VL - 172
SP - 527
EP - 531
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 2
ER -