TY - JOUR
T1 - Leptin in cerebrospinal fluid from children
T2 - Correlation with plasma leptin, sexual dimorphism, and lack of protein binding
AU - Landt, Michael
AU - Parvin, Curtis A.
AU - Wong, Michael
PY - 2000
Y1 - 2000
N2 - Background: Previous studies in adults have established that leptin is present at very low concentrations in cerebrospinal fluid (CSF), but few data exist concerning CSF leptin in children. Current evidence suggests that CSF leptin concentrations interact with hypothalamic centers controlling food intake. Serum leptin concentrations manifest a sexual dimorphism that arises during puberty. Methods: Leptin concentrations were determined in CSF from 42 pre- and postpubertal children who had been objectively classified into non- neurological disease or aseptic meningitis groups. Multivariate analysis of the dependence of CSF leptin on gender, pubertal state, body mass index (BMI), presence of aseptic meningitis, and CSF protein concentration was performed. Results: CSF leptin concentrations correlated with logtransformed plasma leptin concentrations in concomitantly collected samples (r = 0.582; P = 0.029). BMI and gender were significant determinants of CSF leptin in postpubertal children, but only BMI was significant in prepubertal children. Analysis with HPLC to separate protein-bound and free forms of leptin found only free leptin in CSF. Conclusions: CSF leptin concentrations in children reflect plasma leptin concentrations, including the advent of sexual dimorphism at puberty. Only free leptin is detectable in CSF, suggesting that it is the biologically active form. (C) 2000 American Association for Clinical Chemistry.
AB - Background: Previous studies in adults have established that leptin is present at very low concentrations in cerebrospinal fluid (CSF), but few data exist concerning CSF leptin in children. Current evidence suggests that CSF leptin concentrations interact with hypothalamic centers controlling food intake. Serum leptin concentrations manifest a sexual dimorphism that arises during puberty. Methods: Leptin concentrations were determined in CSF from 42 pre- and postpubertal children who had been objectively classified into non- neurological disease or aseptic meningitis groups. Multivariate analysis of the dependence of CSF leptin on gender, pubertal state, body mass index (BMI), presence of aseptic meningitis, and CSF protein concentration was performed. Results: CSF leptin concentrations correlated with logtransformed plasma leptin concentrations in concomitantly collected samples (r = 0.582; P = 0.029). BMI and gender were significant determinants of CSF leptin in postpubertal children, but only BMI was significant in prepubertal children. Analysis with HPLC to separate protein-bound and free forms of leptin found only free leptin in CSF. Conclusions: CSF leptin concentrations in children reflect plasma leptin concentrations, including the advent of sexual dimorphism at puberty. Only free leptin is detectable in CSF, suggesting that it is the biologically active form. (C) 2000 American Association for Clinical Chemistry.
UR - http://www.scopus.com/inward/record.url?scp=0034084843&partnerID=8YFLogxK
U2 - 10.1093/clinchem/46.6.854
DO - 10.1093/clinchem/46.6.854
M3 - Article
C2 - 10839776
AN - SCOPUS:0034084843
SN - 0009-9147
VL - 46
SP - 854
EP - 858
JO - Clinical chemistry
JF - Clinical chemistry
IS - 6
ER -