@article{3521e9417aa144c1b75ee07a193f9598,
title = "Leptin does not directly affect CNS serotonin neurons to influence appetite",
abstract = "Serotonin (5-HT) and leptin play important roles in the modulation of energy balance. Here we investigated mechanisms by which leptin might interact with CNS 5-HT pathways to influence appetite. Although some leptin receptor (LepRb) neurons lie close to 5-HT neurons in the dorsal raphe (DR), 5-HT neurons do not express LepRb. Indeed, while leptin hyperpolarizes some non-5-HT DR neurons, leptin does not alter the activity of DR 5-HT neurons. Furthermore, 5-HT depletion does not impair the anorectic effects of leptin. The serotonin transporter-cre allele (Sertcre) is expressed in 5-HT (and developmentally in some non-5-HT) neurons. While Sertcre promotes LepRb excision in a few LepRb neurons in the hypothalamus, it is not active in DR LepRb neurons, and neuron-specific Sertcre-mediated LepRb inactivation in mice does not alter body weight or adiposity. Thus, leptin does not directly influence 5-HT neurons and does not meaningfully modulate important appetite-related determinants via 5-HT neuron function.",
author = "Lam, {Daniel D.} and Leinninger, {Gina M.} and Louis, {Gwendolyn W.} and Garfield, {Alastair S.} and Marston, {Oliver J.} and Leshan, {Rebecca L.} and Scheller, {Erica L.} and Lyndsay Christensen and Jose Donato and Jing Xia and Evans, {Mark L.} and Carol Elias and Dalley, {Jeffrey W.} and Burdakov, {Denis I.} and Myers, {Martin G.} and Heisler, {Lora K.}",
note = "Funding Information: This work was supported by grants from the Gates Cambridge Trust (D.D.L), the Wellcome Trust WT081713 (L.K.H), the Marilyn H. Vincent Foundation and American Diabetes Association (M.G.M.), NIH (HD061539 to C.E., DK065171 to L.K.H., DK057768 and DK078056 to M.G.M.), and the Cambridge Medical Research Council Centre for Study of Obesity and Related Disorders (L.K.H., D.I.B., and M.L.E.). We thank Drs. Xiaoxi Zhuang and Streamson Chua for the gift of Sert cre and Lepr fl mice, respectively. We thank Amylin Pharmaceuticals for the generous gift of leptin. Core support was provided by NIH DK20572 and DK089503. ",
year = "2011",
month = may,
day = "4",
doi = "10.1016/j.cmet.2011.03.016",
language = "English",
volume = "13",
pages = "584--591",
journal = "Cell Metabolism",
issn = "1550-4131",
number = "5",
}