Lensless wide-field fluorescent imaging on a chip using compressive decoding of sparse objects

Ahmet F. Coskun, Ikbal Sencan, Ting Wei Su, Aydogan Ozean

Research output: Contribution to journalArticlepeer-review

122 Scopus citations


We demonstrate the use of a compressive sampling algorithm for on-chip fluorescent imaging of sparse objects over an ultra-large field-ofview (>8 cm2) without the need for any lenses or mechanical scanning. In this lensfree imaging technique, fluorescent samples placed on a chip are excited through a prism interface, where the pump light is filtered out by total internal reflection after exciting the entire sample volume. The emitted fluorescent light from the specimen is collected through an on-chip fiberoptic faceplate and is delivered to a wide field-of-view opto-electronic sensor array for lensless recording of fluorescent spots corresponding to the samples. A compressive sampling based optimization algorithm is then used to rapidly reconstruct the sparse distribution of fluorescent sources to achieve -10 μm spatial resolution over the entire active region of the sensor-array, i.e., over an imaging field-of-view of >8 cm2. Such a widefield lensless fluorescent imaging platform could especially be significant for high-throughput imaging cytometry, rare cell analysis, as well as for micro-array research.

Original languageEnglish
Pages (from-to)10510-10523
Number of pages14
JournalOptics Express
Issue number10
StatePublished - May 10 2010


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