Lensless fluorescent microscopy on a chip

Ahmet F. Coskun, Ting Wei Su, Ikbal Sencan, Aydogan Ozcan

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

On-chip lensless imaging in general aims to replace bulky lens-based optical microscopes with simpler and more compact designs, especially for high-throughput screening applications. This emerging technology platform has the potential to eliminate the need for bulky and/or costly optical components through the help of novel theories and digital reconstruction algorithms. Along the same lines, here we demonstrate an on-chip fluorescent microscopy modality that can achieve e.g., <4μm spatial resolution over an ultra-wide field-of-view (FOV) of >0.6-8 cm2 without the use of any lenses, mechanical-scanning or thin-film based interference filters. In this technique, fluorescent excitation is achieved through a prism or hemispherical-glass interface illuminated by an incoherent source. After interacting with the entire object volume, this excitation light is rejected by total-internal-reflection (TIR) process that is occurring at the bottom of the sample micro-fluidic chip. The fluorescent emission from the excited objects is then collected by a fiber-optic faceplate or a taper and is delivered to an optoelectronic sensor array such as a charge-coupled-device (CCD). By using a compressive-sampling based decoding algorithm, the acquired lensfree raw fluorescent images of the sample can be rapidly processed to yield e.g., <4μm resolution over an FOV of >0.6-8 cm2. Moreover, vertically stacked micro-channels that are separated by e.g., 50-100 μm can also be successfully imaged using the same lensfree on-chip microscopy platform, which further increases the overall throughput of this modality. This compact on-chip fluorescent imaging platform, with a rapid compressive decoder behind it, could be rather valuable for high-throughput cytometry, rare-cell research and microarray-analysis.

Original languageEnglish
Article numbere3181
JournalJournal of Visualized Experiments
Issue number54
DOIs
StatePublished - Aug 2011

Keywords

  • Bioengineering
  • Compressive sampling/sensing
  • Fluorescent on-chip imaging
  • Issue 54
  • Lensless microoscopy
  • On-chip cytometry
  • Wide-field microscopy

Fingerprint

Dive into the research topics of 'Lensless fluorescent microscopy on a chip'. Together they form a unique fingerprint.

Cite this