Lensfree fluorescent on-chip imaging using compressive sampling

Lens-free fluorescent on-chip imaging using compressive sampling has emerged as a powerful alternative to conventional imaging techniques. An ultrahigh throughput on-chip fluorescent imaging platform has been introduced that can achieve 10-μm resolution over an FOV of >8 cm² without the use of any lenses, thin-film filters, or mechanical scanners. This lens-free fluorescent imaging modality uses a prism-interface to pump the objects-of-interest located within a microfluidic chip, where the excitation light is rejected through total internal reflection occurring at the bottom facet of the substrate. Fluorescent emission from the objects is also collected using a dense fiber-optic faceplate and delivered to a large-format sensor array that has an active area of more than 8 cm².