TY - JOUR
T1 - Leishmania tarentolae taxonomic relatedness inferred from phylogenetic analysis of the small subunit ribosomal RNA gene
AU - Briones, Marcelo R.S.
AU - Nelson, Kimberlyn
AU - Beverley, Stephen M.
AU - Affonso, Heloisa T.
AU - Camargo, Erney P.
AU - Floeter-Winter, Lucile M.
N1 - Funding Information:
This investigation received financial support from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, from UNDP RLA 83, FAPESP (Fundaqao de Amparo fi Pesquisa do Estado de S~.o Paulo, Brazil) and USP-BID (Universidade de Silo Paulo and Banco lnteramericano de Desenvolvimento) to L.M.F.W. Also, M.R.S.B. received a fellowship from FAPESP.
PY - 1992/7
Y1 - 1992/7
N2 - The sequence of the Leishmania tarentolae SSU rRNA (small subunit or 18S rRNA) gene was completely determined from 2 different strains and used to determine phylogenetic relationships between this organism and other trypanosomatids. Extensive structural similarities were observed between L. tarentolae and mammalian leishmanias the SSU rRNA. Phylogenetic reconstructions, using distance matrix or parsimony methods, showed large evolutionary distances between trypanosomes, either African and American, and L. tarentolae. Further analysis using intergenic rDNA spacer (IGS) sequences as probes in dot blot experiments confirmed the results obtained with the SSU rDNA comparisons. The data presented here clearly indicate that L. tarentolae is closely related to the mammalian parasite Leishmania donovani and highly divergent from trypanosomes.
AB - The sequence of the Leishmania tarentolae SSU rRNA (small subunit or 18S rRNA) gene was completely determined from 2 different strains and used to determine phylogenetic relationships between this organism and other trypanosomatids. Extensive structural similarities were observed between L. tarentolae and mammalian leishmanias the SSU rRNA. Phylogenetic reconstructions, using distance matrix or parsimony methods, showed large evolutionary distances between trypanosomes, either African and American, and L. tarentolae. Further analysis using intergenic rDNA spacer (IGS) sequences as probes in dot blot experiments confirmed the results obtained with the SSU rDNA comparisons. The data presented here clearly indicate that L. tarentolae is closely related to the mammalian parasite Leishmania donovani and highly divergent from trypanosomes.
KW - Leishmania tarentolae
KW - SSU rRNA gene
KW - Trypanosomatid phylogeny
UR - http://www.scopus.com/inward/record.url?scp=0026654735&partnerID=8YFLogxK
U2 - 10.1016/0166-6851(92)90014-B
DO - 10.1016/0166-6851(92)90014-B
M3 - Article
C2 - 1501631
AN - SCOPUS:0026654735
SN - 0166-6851
VL - 53
SP - 121
EP - 127
JO - Molecular and Biochemical Parasitology
JF - Molecular and Biochemical Parasitology
IS - 1-2
ER -