Late-stage deuteration of 13C-enriched substrates for: T 1 prolongation in hyperpolarized 13C MRI

Céline Taglang, David E. Korenchan, Cornelius Von Morze, Justin Yu, Chloé Najac, Sinan Wang, Joseph E. Blecha, Sukumar Subramaniam, Robert Bok, Henry F. Vanbrocklin, Daniel B. Vigneron, Sabrina M. Ronen, Renuka Sriram, John Kurhanewicz, David M. Wilson, Robert R. Flavell

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A robust and selective late-stage deuteration methodology was applied to 13C-enriched amino and alpha hydroxy acids to increase spin-lattice relaxation constant T1 for hyperpolarized 13C magnetic resonance imaging. For the five substrates with 13C-labeling on the C1-position ([1-13C]alanine, [1-13C]serine, [1-13C]lactate, [1-13C]glycine, and [1-13C]valine), significant increase of their T1 was observed at 3 T with deuterium labeling (+26%, 22%, +16%, +25% and +29%, respectively). Remarkably, in the case of [2-13C]alanine, [2-13C]serine and [2-13C]lactate, deuterium labeling led to a greater than four fold increase in T1. [1-13C,2-2H]alanine, produced using this method, was applied to in vitro enzyme assays with alanine aminotransferase, demonstrating a kinetic isotope effect.

Original languageEnglish
Pages (from-to)5233-5236
Number of pages4
JournalChemical Communications
Volume54
Issue number41
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

Fingerprint Dive into the research topics of 'Late-stage deuteration of <sup>13</sup>C-enriched substrates for: T <sub>1</sub> prolongation in hyperpolarized <sup>13</sup>C MRI'. Together they form a unique fingerprint.

  • Cite this

    Taglang, C., Korenchan, D. E., Von Morze, C., Yu, J., Najac, C., Wang, S., Blecha, J. E., Subramaniam, S., Bok, R., Vanbrocklin, H. F., Vigneron, D. B., Ronen, S. M., Sriram, R., Kurhanewicz, J., Wilson, D. M., & Flavell, R. R. (2018). Late-stage deuteration of 13C-enriched substrates for: T 1 prolongation in hyperpolarized 13C MRI. Chemical Communications, 54(41), 5233-5236. https://doi.org/10.1039/c8cc02246a