TY - JOUR
T1 - Laser-Initiated Radical Trifluoromethylation of Peptides and Proteins
T2 - Application to Mass-Spectrometry-Based Protein Footprinting
AU - Cheng, Ming
AU - Zhang, Bojie
AU - Cui, Weidong
AU - Gross, Michael L.
N1 - Funding Information:
We are grateful to PMI for the software for data processing, to Prof. Weikai Li for a sample of VKOR, and to the US National Institutes of Health (P41GM103422) for support of this work.
Publisher Copyright:
© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/11/6
Y1 - 2017/11/6
N2 - Described is a novel, laser-initiated radical trifluoromethylation for protein footprinting and its broad residue coverage. .CF3 reacts with 18 of the 20 common amino acids, including Gly, Ala, Ser, Thr, Asp, and Glu, which are relatively silent with regard to .OH. This new approach to footprinting is a bridge between trifluoromethylation in materials and medicinal chemistry and structural biology and biotechnology. Its application to a membrane protein and to myoglobin show that the approach is sensitive to protein conformational change and solvent accessibility.
AB - Described is a novel, laser-initiated radical trifluoromethylation for protein footprinting and its broad residue coverage. .CF3 reacts with 18 of the 20 common amino acids, including Gly, Ala, Ser, Thr, Asp, and Glu, which are relatively silent with regard to .OH. This new approach to footprinting is a bridge between trifluoromethylation in materials and medicinal chemistry and structural biology and biotechnology. Its application to a membrane protein and to myoglobin show that the approach is sensitive to protein conformational change and solvent accessibility.
KW - amino acids
KW - mass spectrometry
KW - membrane proteins
KW - radical reactions
KW - reaction mechanisms
UR - http://www.scopus.com/inward/record.url?scp=85030553460&partnerID=8YFLogxK
U2 - 10.1002/anie.201706697
DO - 10.1002/anie.201706697
M3 - Article
C2 - 28901679
AN - SCOPUS:85030553460
SN - 1433-7851
VL - 56
SP - 14007
EP - 14010
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 45
ER -