Laser-Initiated Radical Trifluoromethylation of Peptides and Proteins: Application to Mass-Spectrometry-Based Protein Footprinting

Ming Cheng, Bojie Zhang, Weidong Cui, Michael L. Gross

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Described is a novel, laser-initiated radical trifluoromethylation for protein footprinting and its broad residue coverage. .CF3 reacts with 18 of the 20 common amino acids, including Gly, Ala, Ser, Thr, Asp, and Glu, which are relatively silent with regard to .OH. This new approach to footprinting is a bridge between trifluoromethylation in materials and medicinal chemistry and structural biology and biotechnology. Its application to a membrane protein and to myoglobin show that the approach is sensitive to protein conformational change and solvent accessibility.

Original languageEnglish
Pages (from-to)14007-14010
Number of pages4
JournalAngewandte Chemie - International Edition
Volume56
Issue number45
DOIs
StatePublished - Nov 6 2017

Keywords

  • amino acids
  • mass spectrometry
  • membrane proteins
  • radical reactions
  • reaction mechanisms

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