Large-scale rewiring of innate immunity circuitry and microRNA regulation during initial rice blast infection

Ze Yuan Li, Jing Xia, Zheng Chen, Yang Yu, Quan Feng Li, Yu Chan Zhang, Jin Ping Zhang, Cong Ying Wang, Xiao Yuan Zhu, Weixiong Zhang, Yue Qin Chen

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Rice blast is a recurrent fungal disease, and resistance to fungal infection is a complex trait. Therefore, a comprehensive examination of rice transcriptome and its variation during fungal infection is necessary to understand the complex gene regulatory networks. In this study, adopting Next-Generation Sequencing we profiled the transcriptomes and microRNAomes of rice varieties, one susceptible and the other resistant to M. oryzae, at multiple time points during the fungal infection. Our results revealed a substantial variation in the plant transcriptome and microRNAome as well as change to rice innate immunity during fungal infection. A number of putative R gene candidates were identified from a perturbed rice transcriptome analysis. The expression of genes and non-coding RNA molecules changed in both fungal resistant and susceptible plants during M. oryzae invasion discovered distinct pathways triggered in the susceptible and resistant plants. In addition, a number of fungus genes in the susceptible and resistant plants were constantly expressed at different time points, suggesting that they were likely to be the potential AVR genes. Our results revealed large-scale rewiring of innate immunity circuitry and microRNA regulation during initial rice blast infection, which would help to develop more robust blast-resistant rice plants.

Original languageEnglish
Article number25493
JournalScientific reports
Volume6
DOIs
StatePublished - May 6 2016

Fingerprint

Dive into the research topics of 'Large-scale rewiring of innate immunity circuitry and microRNA regulation during initial rice blast infection'. Together they form a unique fingerprint.

Cite this