Large-scale global identification of protein lysine methylation in vivo

Xing Jun Cao, Anna M. Arnaudo, Benjamin A. Garcia

Research output: Contribution to journalArticlepeer-review

103 Scopus citations

Abstract

Lysine methylation mediated by methyltransferase enzymes is present on multiple proteins throughout the cell; however, methods to uncover and characterize global protein lysine methylation patterns do not readily exist. Here we developed pan-specific methyl lysine antibodies that we utilized in immunoprecipitation experiments coupled with mass spectrometry to yield one of the first large-scale surveys of protein lysine methylation in vivo. In total, 552 different lysine methylation sites were determined, making this one of the most comprehensive global studies published to date. The large majority of these sites have not been yet reported. These sites showed significantly enriched sequence motifs and resided in proteins that are involved in diverse biological processes, particularly in chromatin organization. Our data provide a comprehensive view of lysine methylation in human cells and a powerful resource to facilitate investigations into the function of lysine methylation on non-histone proteins.

Original languageEnglish
Pages (from-to)477-485
Number of pages9
JournalEpigenetics
Volume8
Issue number5
DOIs
StatePublished - May 2013

Keywords

  • Affinity
  • Histone
  • Immunoprecipitation
  • Mass spectrometry
  • Methylation
  • Non-histone
  • Proteomics

Fingerprint

Dive into the research topics of 'Large-scale global identification of protein lysine methylation in vivo'. Together they form a unique fingerprint.

Cite this