Laminin compensation in collagen α3(IV) knockout (Alport) glomeruli contributes to permeability defects

Dale R. Abrahamson, Kathryn Isom, Eileen Roach, Larysa Stroganova, Adrian Zelenchuk, Jeffrey H. Miner, Patricia L. St. John

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Alport disease is caused by mutations in genes encoding the α3, α4, or α5 chains of type IV collagen, which form the collagenous network of mature glomerular basement membrane (GBM). In the absence of α3, α4, α5 (IV) collagen, α1, α2 (IV) collagen persists, which ordinarily is found only in GBM of developing kidney. In addition to dysregulation of collagen IV, Alport GBM contains aberrant laminins, which may contribute to the progressive GBM thickening and splitting, proteinuria, and renal failure seen in this disorder. This study sought to characterize further the laminin dysregulation in collagen α3(IV) knockout mice, a model of Alport disease. With the use of confocal microscopy, laminin α1 and α5 abundance was quantified, and it was found that they co-distributed in significantly large amounts in areas of GBM thickening. In addition, labeling of entire glomeruli for laminin α5 was significantly greater in Alport mice than in wild-type siblings. Reverse transcriptase-PCR from isolated glomeruli demonstrated significantly more laminin α5 mRNA in Alport mice than in wild-type controls, indicating upregulated transcription of Lama5. For testing glomerular barrier function, ferritin was injected into 2-wk-old Alport and control mice, and GBM was examined by electron microscopy. Highest ferritin levels were seen in Alport GBM thickenings beneath effaced podocyte foot processes, but morphologically normal GBM was significantly permeable as well. We concluded that (1) ultrastructurally normal Alport GBM residing beneath differentiated podocyte foot processes is inherently and abnormally permeable, and (2) upregulation of Lama5 transcription and concentration of laminin α1 and α5 within Alport GBM thickenings contribute to abnormal permeabilities.

Original languageEnglish
Pages (from-to)2465-2472
Number of pages8
JournalJournal of the American Society of Nephrology
Volume18
Issue number9
DOIs
StatePublished - Sep 1 2007

Fingerprint Dive into the research topics of 'Laminin compensation in collagen α3(IV) knockout (Alport) glomeruli contributes to permeability defects'. Together they form a unique fingerprint.

  • Cite this