Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis

Jing Gao, Mindy C. DeRouen, Chih Hsin Chen, Michael Nguyen, Ngon T. Nguyen, Hiroyuki Ido, Kenji Harada, Kiyotoshi Sekiguchi, Bruce A. Morgan, Jeffery H. Miner, Anthony E. Oro, M. Peter Marinkovich

Research output: Contribution to journalArticlepeer-review

99 Scopus citations


Hair morphogenesis takes place through reciprocal epithelial and mesenchymal signaling; however, the mechanisms controlling signal exchange are poorly understood. Laminins are extracellular proteins that play critical roles in adhesion and signaling. Here we demonstrate the mechanism of how laminin-511 controls hair morphogenesis. Dermal papilla (DP) from laminin-511 mutants showed developmental defects by E16.5, including a failure to maintain expression of the key morphogen noggin. This maintenance was critical as exogenous introduction of noggin or sonic hedgehog (Shh) produced downstream from noggin was sufficient to restore hair follicle development in lama5-/- (laminin-511-null) skin. Hair development required the β1 integrin binding but not the heparin binding domain of laminin-511. Previous studies demonstrated that Shh signaling requires primary cilia, microtubule-based signaling organelles. Laminin-511 mutant DP showed decreased length and structure of primary cilia in vitro and in vivo. Laminin-511, but not laminin-111, restored primary cilia formation in lama5-/- mesenchyme and triggered noggin expression in an Shh- and PDGF-dependent manner. Inhibition of laminin-511 receptor β1 integrin disrupted DP primary cilia formation as well as hair development. These studies show that epithelial-derived laminin-511 is a critical early signal that directs ciliary function and DP maintenance as a requirement for hair follicle downgrowth.

Original languageEnglish
Pages (from-to)2111-2124
Number of pages14
JournalGenes and Development
Issue number15
StatePublished - Aug 1 2008


  • Basement membrane
  • Hair
  • Integrin
  • Laminin
  • Primary cilium


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