TY - JOUR
T1 - Lactate dehydrogenase release is facilitated by brief sonication of rat hippocampal slices and isolated retinas following acute neuronal damage
AU - Izumi, Y.
AU - Izumi, M.
AU - Benz, A. M.
AU - Zorumski, C. F.
N1 - Funding Information:
This work was supported by the Washington University Clinical Nutrition Research Unit Center Grant (DK56341), grants MH45493 and AG11355 from NIH, and the Bantly Foundation.
PY - 2001/7/15
Y1 - 2001/7/15
N2 - Although useful for determining neuronal damage in cell cultures, the lactate dehydrogenase (LDH) assay is not suitable for acute brain preparations because LDH release is typically delayed relative to neuronal deterioration. The slow release suggests that LDH may remain trapped inside damaged cells until late in the degenerative process. To test this, we examined whether brief sonication facilitates LDH release from acutely damaged neurons. In rat isolated retinas and hippocampal slices, LDH release was minimal following acute administration of iodoacetate or kainate. However, these toxins promoted significant LDH release, when toxin exposure was followed by brief sonication. Increases in extracellular LDH correlated with changes in neuronal morphology. These findings suggest that sonication may facilitate the use of the LDH assay in acute brain preparations.
AB - Although useful for determining neuronal damage in cell cultures, the lactate dehydrogenase (LDH) assay is not suitable for acute brain preparations because LDH release is typically delayed relative to neuronal deterioration. The slow release suggests that LDH may remain trapped inside damaged cells until late in the degenerative process. To test this, we examined whether brief sonication facilitates LDH release from acutely damaged neurons. In rat isolated retinas and hippocampal slices, LDH release was minimal following acute administration of iodoacetate or kainate. However, these toxins promoted significant LDH release, when toxin exposure was followed by brief sonication. Increases in extracellular LDH correlated with changes in neuronal morphology. These findings suggest that sonication may facilitate the use of the LDH assay in acute brain preparations.
KW - Hippocampus
KW - Lactate dehydrogenase (LDH)
KW - Neuronal degeneration
KW - Retina
UR - http://www.scopus.com/inward/record.url?scp=0035878598&partnerID=8YFLogxK
U2 - 10.1016/S0165-0270(01)00369-7
DO - 10.1016/S0165-0270(01)00369-7
M3 - Article
C2 - 11459617
AN - SCOPUS:0035878598
SN - 0165-0270
VL - 108
SP - 49
EP - 55
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 1
ER -