Lack of Evidence That Male Fetal Microchimerism is Present in Endometriosis

Amelie Fassbender; Maria Debiec-Rychter; Rieta Van Bree; Joris Robert Vermeesch; Christel Meuleman; Carla Tomassetti; Karen Peeraer; Thomas D'hooghe; Dan I. Lebovic

doi:10.1177/1933719115574343

Lack of Evidence That Male Fetal Microchimerism is Present in Endometriosis

Amelie Fassbender, Maria Debiec-Rychter, Rieta Van Bree, Joris Robert Vermeesch, Christel Meuleman, Carla Tomassetti, Karen Peeraer, Thomas D'hooghe, Dan I. Lebovic

Division of Reproductive Endocrinology & Infertility

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Introduction: Fetal microchimerism has been implicated in the etiology of autoimmune diseases. This study was done to test the hypothesis that male fetal microchimerism is present in eutopic and ectopic endometrium (EM) obtained from women with endometriosis but not in eutopic EM from women without endometriosis. Methods: A total of 31 patients were selected, including women with endometriosis (paired eutopic and ectopic EM; n = 19) and women without endometriosis (eutopic EM; n = 12). Tricolor interphase fluorescence in situ hybridization analysis was performed by cohybridization of CEP Y SpectrumAqua and CEP X SpectrumGreen (SG)/CEP Y SpectrumOrange probes. Results: Ectopic EM from women with endometriosis had 75% XX chromosomes (double SG signals) and 25% X chromosomes (single SG signal). Y chromosomes were not observed in any of the eutopic/ectopic endometrial tissues from cases or controls. Conclusions: We were unable to confirm our hypothesis that male fetal microchimerism is present in eutopic and/or ectopic EM obtained from women with endometriosis.

Original language	English
Pages (from-to)	1115-1121
Number of pages	7
Journal	Reproductive Sciences
Volume	22
Issue number	9
DOIs	https://doi.org/10.1177/1933719115574343
State	Published - Sep 12 2015

Keywords

FISH
endometrium
male fetal microchimerism

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10.1177/1933719115574343

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@article{311ba89754674e0183c54cca19affcae,

title = "Lack of Evidence That Male Fetal Microchimerism is Present in Endometriosis",

abstract = "Introduction: Fetal microchimerism has been implicated in the etiology of autoimmune diseases. This study was done to test the hypothesis that male fetal microchimerism is present in eutopic and ectopic endometrium (EM) obtained from women with endometriosis but not in eutopic EM from women without endometriosis. Methods: A total of 31 patients were selected, including women with endometriosis (paired eutopic and ectopic EM; n = 19) and women without endometriosis (eutopic EM; n = 12). Tricolor interphase fluorescence in situ hybridization analysis was performed by cohybridization of CEP Y SpectrumAqua and CEP X SpectrumGreen (SG)/CEP Y SpectrumOrange probes. Results: Ectopic EM from women with endometriosis had 75% XX chromosomes (double SG signals) and 25% X chromosomes (single SG signal). Y chromosomes were not observed in any of the eutopic/ectopic endometrial tissues from cases or controls. Conclusions: We were unable to confirm our hypothesis that male fetal microchimerism is present in eutopic and/or ectopic EM obtained from women with endometriosis.",

keywords = "FISH, endometrium, male fetal microchimerism",

author = "Amelie Fassbender and Maria Debiec-Rychter and Bree, {Rieta Van} and Vermeesch, {Joris Robert} and Christel Meuleman and Carla Tomassetti and Karen Peeraer and Thomas D'hooghe and Lebovic, {Dan I.}",

note = "Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported in part by the National Institutes of Health Grant 5K23HD043952-02 (to Prof. Dan Lebovic). This study was supported by grants from the Leuven University Hospital Fund and the Department of Obstetrics and Gynecology, University of Wisconsin School of Medicine and Public Health. Publisher Copyright: {\textcopyright} The Author(s) 2015.",

year = "2015",

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doi = "10.1177/1933719115574343",

language = "English",

volume = "22",

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journal = "Reproductive Sciences",

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T1 - Lack of Evidence That Male Fetal Microchimerism is Present in Endometriosis

AU - Fassbender, Amelie

AU - Debiec-Rychter, Maria

AU - Bree, Rieta Van

AU - Vermeesch, Joris Robert

AU - Meuleman, Christel

AU - Tomassetti, Carla

AU - Peeraer, Karen

AU - D'hooghe, Thomas

AU - Lebovic, Dan I.

N1 - Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported in part by the National Institutes of Health Grant 5K23HD043952-02 (to Prof. Dan Lebovic). This study was supported by grants from the Leuven University Hospital Fund and the Department of Obstetrics and Gynecology, University of Wisconsin School of Medicine and Public Health. Publisher Copyright: © The Author(s) 2015.

PY - 2015/9/12

Y1 - 2015/9/12

N2 - Introduction: Fetal microchimerism has been implicated in the etiology of autoimmune diseases. This study was done to test the hypothesis that male fetal microchimerism is present in eutopic and ectopic endometrium (EM) obtained from women with endometriosis but not in eutopic EM from women without endometriosis. Methods: A total of 31 patients were selected, including women with endometriosis (paired eutopic and ectopic EM; n = 19) and women without endometriosis (eutopic EM; n = 12). Tricolor interphase fluorescence in situ hybridization analysis was performed by cohybridization of CEP Y SpectrumAqua and CEP X SpectrumGreen (SG)/CEP Y SpectrumOrange probes. Results: Ectopic EM from women with endometriosis had 75% XX chromosomes (double SG signals) and 25% X chromosomes (single SG signal). Y chromosomes were not observed in any of the eutopic/ectopic endometrial tissues from cases or controls. Conclusions: We were unable to confirm our hypothesis that male fetal microchimerism is present in eutopic and/or ectopic EM obtained from women with endometriosis.

AB - Introduction: Fetal microchimerism has been implicated in the etiology of autoimmune diseases. This study was done to test the hypothesis that male fetal microchimerism is present in eutopic and ectopic endometrium (EM) obtained from women with endometriosis but not in eutopic EM from women without endometriosis. Methods: A total of 31 patients were selected, including women with endometriosis (paired eutopic and ectopic EM; n = 19) and women without endometriosis (eutopic EM; n = 12). Tricolor interphase fluorescence in situ hybridization analysis was performed by cohybridization of CEP Y SpectrumAqua and CEP X SpectrumGreen (SG)/CEP Y SpectrumOrange probes. Results: Ectopic EM from women with endometriosis had 75% XX chromosomes (double SG signals) and 25% X chromosomes (single SG signal). Y chromosomes were not observed in any of the eutopic/ectopic endometrial tissues from cases or controls. Conclusions: We were unable to confirm our hypothesis that male fetal microchimerism is present in eutopic and/or ectopic EM obtained from women with endometriosis.

KW - FISH

KW - endometrium

KW - male fetal microchimerism

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ER -

Lack of Evidence That Male Fetal Microchimerism is Present in Endometriosis

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