Lack of acidification in Mycobacterium phagosomes produced by exclusion of the vesicular proton-ATPase

Sheila Sturgill-Koszycki, Paul H. Schlesinger, Prasanta Chakraborty, Pryce L. Haddix, Helen L. Collins, Agnes K. Fok, Richard D. Allen, Stephen L. Gluck, John Heuser, David G. Russell

Research output: Contribution to journalArticlepeer-review

991 Scopus citations

Abstract

The success of Mycobacterium species as pathogens depends on their ability to maintain an infection inside the phagocytic vacuole of the macrophage. Although the bacteria are reported to modulate maturation of their intracellular vacuoles, the nature of such modifications is unknown. In this study, vacuoles formed around Mycobacterium avium failed to acidify below pH 6.3 to 6.5. Immunoelectron microscopy of infected macrophages and immunoblotting of isolated phagosomes showed that Mycobacterium vacuoles acquire the lysosomal membrane protein LAMP-1, but not the vesicular proton - adenosine triphosphatase (ATPase) responsible for phagosomal acidification. This suggests either a selective inhibition of fusion with proton-ATPase - containing vesicles or a rapid removal of the complex from Mycobacterium phagosomes.

Original languageEnglish
Pages (from-to)678-681
Number of pages4
JournalScience
Volume263
Issue number5147
DOIs
StatePublished - 1994

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