TY - JOUR
T1 - Laboratory detection of vancomycin nonsusceptible staphylococcus aureus
AU - Burnham, Carey Ann D.
AU - Craven, Robin R.
AU - Dunne, W. Michael
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - In 2009, the Clinical and Laboratory Standards Institute introduced new guidelines for staphylococcal susceptibility testing, indicating that Kirby Bauer disk diffusion is not a reliable method for detection of reduced susceptibility to vancomycin in this genus. In this unit, we describe a method using a brain-heart infusion medium containing 3 μg/ml of vancomycin (BHI-V3) to screen for isolates of S. aureus with reduced susceptibility to vancomycin; this medium represents a sensitive and cost-effective method for detection of such isolates in the clinical or research laboratory.
AB - In 2009, the Clinical and Laboratory Standards Institute introduced new guidelines for staphylococcal susceptibility testing, indicating that Kirby Bauer disk diffusion is not a reliable method for detection of reduced susceptibility to vancomycin in this genus. In this unit, we describe a method using a brain-heart infusion medium containing 3 μg/ml of vancomycin (BHI-V3) to screen for isolates of S. aureus with reduced susceptibility to vancomycin; this medium represents a sensitive and cost-effective method for detection of such isolates in the clinical or research laboratory.
KW - Screening agar
KW - Staphylococcus aureus
KW - Susceptibility testing
KW - Vancomycin
KW - Vancomycin-intermediate Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=84857654819&partnerID=8YFLogxK
U2 - 10.1002/9780471729259.mc1706s22
DO - 10.1002/9780471729259.mc1706s22
M3 - Article
AN - SCOPUS:84857654819
JO - Current Protocols in Microbiology
JF - Current Protocols in Microbiology
SN - 1934-8525
IS - SUPPL. 22
ER -