Laboratory detection of vancomycin nonsusceptible staphylococcus aureus

Carey Ann D. Burnham, Robin R. Craven, W. Michael Dunne

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


In 2009, the Clinical and Laboratory Standards Institute introduced new guidelines for staphylococcal susceptibility testing, indicating that Kirby Bauer disk diffusion is not a reliable method for detection of reduced susceptibility to vancomycin in this genus. In this unit, we describe a method using a brain-heart infusion medium containing 3 μg/ml of vancomycin (BHI-V3) to screen for isolates of S. aureus with reduced susceptibility to vancomycin; this medium represents a sensitive and cost-effective method for detection of such isolates in the clinical or research laboratory.

Original languageEnglish
JournalCurrent Protocols in Microbiology
Issue numberSUPPL. 22
StatePublished - 2011


  • Screening agar
  • Staphylococcus aureus
  • Susceptibility testing
  • Vancomycin
  • Vancomycin-intermediate Staphylococcus aureus


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