TY - JOUR
T1 - Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP
AU - Moriyoshi, Koki
AU - Richards, Linda J.
AU - Akazawa, Chihiro
AU - O'Leary, Dennis D.M.
AU - Nakanishi, Shigetada
N1 - Funding Information:
We thank M. Chalfie and Y. Mishina for GFP clones, I. Saito for the original adenovirus and helpful advice on constructing recombinant viruses, J. Miyazaki for the CAG promoter, T. Sakaguchi for the slice culture technique, T. Minoshima for preparing cortical cultures, P. Yates for work on video imaging and analysis, D. Peterson and T. Palmer for help with confocal imaging, J. Boulter and S. Choe for technical advice, and S. Boutsaboualoy for technical assistance. This work was supported in part by research grants from the Ministry of Education, Science and Culture of Japan, the Sankyo Foundation, National Institutes of Health grant NS 31558, The March of Dimes, and the Lucille P. Markey Charitable Trust. L. J. R. is a Lucille P. Markey Visiting Fellow.
PY - 1996/2
Y1 - 1996/2
N2 - We describe an experimental system to visualize the soma and processes of mammalian neurons and glia in living and fixed preparations by using a recombinant adenovirus vector to transfer the jellyfish green fluorescent protein (GFP) into postmitotic neural cells both in vitro and in vivo. We have introduced several modifications of GFP that enhance its fluorescence intensity in mammalian axons and dendrites. This method should be useful for studying the dynamic processes of cell migration and the development of neuronal connections, as well as for analyzing the function of exogenous genes introduced into cells using the adenovirus vector.
AB - We describe an experimental system to visualize the soma and processes of mammalian neurons and glia in living and fixed preparations by using a recombinant adenovirus vector to transfer the jellyfish green fluorescent protein (GFP) into postmitotic neural cells both in vitro and in vivo. We have introduced several modifications of GFP that enhance its fluorescence intensity in mammalian axons and dendrites. This method should be useful for studying the dynamic processes of cell migration and the development of neuronal connections, as well as for analyzing the function of exogenous genes introduced into cells using the adenovirus vector.
UR - http://www.scopus.com/inward/record.url?scp=0030048198&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(00)80044-6
DO - 10.1016/S0896-6273(00)80044-6
M3 - Article
C2 - 8789941
AN - SCOPUS:0030048198
SN - 0896-6273
VL - 16
SP - 255
EP - 260
JO - Neuron
JF - Neuron
IS - 2
ER -