Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP

Koki Moriyoshi, Linda J. Richards, Chihiro Akazawa, Dennis D.M. O'Leary, Shigetada Nakanishi

Research output: Contribution to journalArticlepeer-review

248 Scopus citations

Abstract

We describe an experimental system to visualize the soma and processes of mammalian neurons and glia in living and fixed preparations by using a recombinant adenovirus vector to transfer the jellyfish green fluorescent protein (GFP) into postmitotic neural cells both in vitro and in vivo. We have introduced several modifications of GFP that enhance its fluorescence intensity in mammalian axons and dendrites. This method should be useful for studying the dynamic processes of cell migration and the development of neuronal connections, as well as for analyzing the function of exogenous genes introduced into cells using the adenovirus vector.

Original languageEnglish
Pages (from-to)255-260
Number of pages6
JournalNeuron
Volume16
Issue number2
DOIs
StatePublished - Feb 1996

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