Label-retention expansion microscopy

Xiaoyu Shi; Qi Li; Zhipeng Dai; Arthur A. Tran; Siyu Feng; Alejandro D. Ramirez; Zixi Lin; Xiaomeng Wang; Tracy T. Chow; Jiapei Chen; Dhivya Kumar; Andrew R. McColloch; Jeremy F. Reiter; Eric J. Huang; Ian B. Seiple; Bo Huang

doi:10.1083/jcb.202105067

Label-retention expansion microscopy

Xiaoyu Shi, Qi Li, Zhipeng Dai, Arthur A. Tran, Siyu Feng, Alejandro D. Ramirez, Zixi Lin, Xiaomeng Wang, Tracy T. Chow, Jiapei Chen, Dhivya Kumar, Andrew R. McColloch, Jeremy F. Reiter, Eric J. Huang, Ian B. Seiple, Bo Huang

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46 Scopus citations

Abstract

Expansion microscopy (ExM) increases the effective resolving power of any microscope by expanding the sample with swellable hydrogel. Since its invention, ExM has been successfully applied to a wide range of cell, tissue, and animal samples. Still, fluorescence signal loss during polymerization and digestion limits molecular-scale imaging using ExM. Here, we report the development of label-retention ExM (LR-ExM) with a set of trifunctional anchors that not only prevent signal loss but also enable high-efficiency labeling using SNAP and CLIP tags. We have demonstrated multicolor LR-ExM for a variety of subcellular structures. Combining LR-ExM with superresolution stochastic optical reconstruction microscopy (STORM), we have achieved molecular resolution in the visualization of polyhedral lattice of clathrin-coated pits in situ.

Original language	English
Article number	e202105067
Journal	Journal of Cell Biology
Volume	220
Issue number	9
DOIs	https://doi.org/10.1083/jcb.202105067
State	Published - 2021

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title = "Label-retention expansion microscopy",

abstract = "Expansion microscopy (ExM) increases the effective resolving power of any microscope by expanding the sample with swellable hydrogel. Since its invention, ExM has been successfully applied to a wide range of cell, tissue, and animal samples. Still, fluorescence signal loss during polymerization and digestion limits molecular-scale imaging using ExM. Here, we report the development of label-retention ExM (LR-ExM) with a set of trifunctional anchors that not only prevent signal loss but also enable high-efficiency labeling using SNAP and CLIP tags. We have demonstrated multicolor LR-ExM for a variety of subcellular structures. Combining LR-ExM with superresolution stochastic optical reconstruction microscopy (STORM), we have achieved molecular resolution in the visualization of polyhedral lattice of clathrin-coated pits in situ.",

author = "Xiaoyu Shi and Qi Li and Zhipeng Dai and Tran, {Arthur A.} and Siyu Feng and Ramirez, {Alejandro D.} and Zixi Lin and Xiaomeng Wang and Chow, {Tracy T.} and Jiapei Chen and Dhivya Kumar and McColloch, {Andrew R.} and Reiter, {Jeremy F.} and Huang, {Eric J.} and Seiple, {Ian B.} and Bo Huang",

note = "Publisher Copyright: {\textcopyright} 2021 Shi et al.",

year = "2021",

doi = "10.1083/jcb.202105067",

language = "English",

volume = "220",

journal = "Journal of Cell Biology",

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TY - JOUR

T1 - Label-retention expansion microscopy

AU - Shi, Xiaoyu

AU - Li, Qi

AU - Dai, Zhipeng

AU - Tran, Arthur A.

AU - Feng, Siyu

AU - Ramirez, Alejandro D.

AU - Lin, Zixi

AU - Wang, Xiaomeng

AU - Chow, Tracy T.

AU - Chen, Jiapei

AU - Kumar, Dhivya

AU - McColloch, Andrew R.

AU - Reiter, Jeremy F.

AU - Huang, Eric J.

AU - Seiple, Ian B.

AU - Huang, Bo

PY - 2021

Y1 - 2021

N2 - Expansion microscopy (ExM) increases the effective resolving power of any microscope by expanding the sample with swellable hydrogel. Since its invention, ExM has been successfully applied to a wide range of cell, tissue, and animal samples. Still, fluorescence signal loss during polymerization and digestion limits molecular-scale imaging using ExM. Here, we report the development of label-retention ExM (LR-ExM) with a set of trifunctional anchors that not only prevent signal loss but also enable high-efficiency labeling using SNAP and CLIP tags. We have demonstrated multicolor LR-ExM for a variety of subcellular structures. Combining LR-ExM with superresolution stochastic optical reconstruction microscopy (STORM), we have achieved molecular resolution in the visualization of polyhedral lattice of clathrin-coated pits in situ.

AB - Expansion microscopy (ExM) increases the effective resolving power of any microscope by expanding the sample with swellable hydrogel. Since its invention, ExM has been successfully applied to a wide range of cell, tissue, and animal samples. Still, fluorescence signal loss during polymerization and digestion limits molecular-scale imaging using ExM. Here, we report the development of label-retention ExM (LR-ExM) with a set of trifunctional anchors that not only prevent signal loss but also enable high-efficiency labeling using SNAP and CLIP tags. We have demonstrated multicolor LR-ExM for a variety of subcellular structures. Combining LR-ExM with superresolution stochastic optical reconstruction microscopy (STORM), we have achieved molecular resolution in the visualization of polyhedral lattice of clathrin-coated pits in situ.

UR - http://www.scopus.com/inward/record.url?scp=85110976960&partnerID=8YFLogxK

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DO - 10.1083/jcb.202105067

M3 - Article

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SN - 0021-9525

VL - 220

JO - Journal of Cell Biology

JF - Journal of Cell Biology

IS - 9

M1 - e202105067

ER -

Label-retention expansion microscopy

Abstract

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