@inbook{ee0dad3cab4c4e869f81cc70b21ed2fc,
title = "Label-free quantitation for clinical proteomics",
abstract = "Label-free quantification (LFQ) has emerged as a viable option for quantitative LC-MS/MS-based proteomic analyses for use on the scale of hundreds of samples such as are encountered in clinical analysis. Notably, sample preparation, sample loading, HPLC separations, and mass spectrometric performance must be highly reproducible for this approach to be effective. The following protocols describe the key steps in the methods related to sample preparation and analysis for LC-MS/MS-based label-free quantitation using standard data-dependent acquisition.",
keywords = "Area under the curve (AUC), Label-free quantification, Mass spectrometry, Proteomics",
author = "Robert Moulder and Goo, {Young Ah} and Goodlett, {David R.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
month = feb,
day = "1",
doi = "10.1007/978-1-4939-3524-6_4",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "65--76",
booktitle = "Methods in Molecular Biology",
}