Label-free protein recognition two-dimensional array using nanomechanical sensors

Min Yue; Jeanne C. Stachowiak; Henry Lin; Ram Datar; Richard Cote; Arun Majumdar

doi:10.1021/nl072740c

Label-free protein recognition two-dimensional array using nanomechanical sensors

Min Yue, Jeanne C. Stachowiak, Henry Lin, Ram Datar, Richard Cote, Arun Majumdar

Research output: Contribution to journal › Article › peer-review

110 Scopus citations

Abstract

We demonstrate two-dimensional multiplexed real-time, label-free antibody-antigen binding assays by optically detecting nanoscale motions of two-dimensional arrays of microcantilever beams. Prostate specific antigen (PSA) was assayed using antibodies covalently bound to one surface of the cantilevers by two different surface chemistries, while the nonreaction surfaces were passivated by polyethylene glycol)-silane. PSA as low as 1 ng/mL was detected while 2 mg/μl of bovine serum albumin induced only negligible deflection on the cantilevers.

Original language	English
Pages (from-to)	520-524
Number of pages	5
Journal	Nano Letters
Volume	8
Issue number	2
DOIs	https://doi.org/10.1021/nl072740c
State	Published - Feb 1 2008

Access to Document

10.1021/nl072740c

Cite this

@article{7fe2d5ef316d47cb967c5b33ec18bb21,

title = "Label-free protein recognition two-dimensional array using nanomechanical sensors",

abstract = "We demonstrate two-dimensional multiplexed real-time, label-free antibody-antigen binding assays by optically detecting nanoscale motions of two-dimensional arrays of microcantilever beams. Prostate specific antigen (PSA) was assayed using antibodies covalently bound to one surface of the cantilevers by two different surface chemistries, while the nonreaction surfaces were passivated by polyethylene glycol)-silane. PSA as low as 1 ng/mL was detected while 2 mg/μl of bovine serum albumin induced only negligible deflection on the cantilevers.",

author = "Min Yue and Stachowiak, {Jeanne C.} and Henry Lin and Ram Datar and Richard Cote and Arun Majumdar",

year = "2008",

month = feb,

day = "1",

doi = "10.1021/nl072740c",

language = "English",

volume = "8",

pages = "520--524",

journal = "Nano Letters",

issn = "1530-6984",

number = "2",

}

TY - JOUR

T1 - Label-free protein recognition two-dimensional array using nanomechanical sensors

AU - Yue, Min

AU - Stachowiak, Jeanne C.

AU - Lin, Henry

AU - Datar, Ram

AU - Cote, Richard

AU - Majumdar, Arun

PY - 2008/2/1

Y1 - 2008/2/1

N2 - We demonstrate two-dimensional multiplexed real-time, label-free antibody-antigen binding assays by optically detecting nanoscale motions of two-dimensional arrays of microcantilever beams. Prostate specific antigen (PSA) was assayed using antibodies covalently bound to one surface of the cantilevers by two different surface chemistries, while the nonreaction surfaces were passivated by polyethylene glycol)-silane. PSA as low as 1 ng/mL was detected while 2 mg/μl of bovine serum albumin induced only negligible deflection on the cantilevers.

AB - We demonstrate two-dimensional multiplexed real-time, label-free antibody-antigen binding assays by optically detecting nanoscale motions of two-dimensional arrays of microcantilever beams. Prostate specific antigen (PSA) was assayed using antibodies covalently bound to one surface of the cantilevers by two different surface chemistries, while the nonreaction surfaces were passivated by polyethylene glycol)-silane. PSA as low as 1 ng/mL was detected while 2 mg/μl of bovine serum albumin induced only negligible deflection on the cantilevers.

UR - http://www.scopus.com/inward/record.url?scp=40449105434&partnerID=8YFLogxK

U2 - 10.1021/nl072740c

DO - 10.1021/nl072740c

M3 - Article

C2 - 18179277

AN - SCOPUS:40449105434

SN - 1530-6984

VL - 8

SP - 520

EP - 524

JO - Nano Letters

JF - Nano Letters

IS - 2

ER -

Label-free protein recognition two-dimensional array using nanomechanical sensors

Abstract

Access to Document

Other files and links

Fingerprint

Cite this