Label-free, multiplexed detection of bacterial tmRNA using silicon photonic microring resonators

Ott Scheler, Jared T. Kindt, Abraham J. Qavi, Lauris Kaplinski, Barry Glynn, Thomas Barry, Ants Kurg, Ryan C. Bailey

Research output: Contribution to journalArticlepeer-review

66 Scopus citations


A label-free biosensing method for the sensitive detection and identification of bacterial transfer-messenger RNA (tmRNA) is presented employing arrays of silicon photonic microring resonators. Species specific tmRNA molecules are targeted by complementary DNA capture probes that are covalently attached to the sensor surface. Specific hybridization is monitored in near real-time by observing the resonance wavelength shift of each individual microring. The sensitivity of the biosensing platform allowed for detection down to 53fmol of Streptococcus pneumoniae tmRNA, equivalent to approximately 3.16×10 7 CFU of bacteria. The simplicity and scalability of this biosensing approach makes it a promising tool for the rapid identification of different bacteria via tmRNA profiling.

Original languageEnglish
Pages (from-to)56-61
Number of pages6
JournalBiosensors and Bioelectronics
Issue number1
StatePublished - Jun 2012


  • Bacterial detection
  • Biomarker
  • Label-free biosensing
  • Microring resonator
  • Silicon photonics
  • TmRNA


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