Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes

Fumiaki N. Ishikawa; Hsiao Kang Chang; Marco Curreli; Hsiang I. Liao; C. Anders Olson; Po Chiang Chen; Rui Zhang; Richard W. Roberts; Ren Sun; Richard J. Cote; Mark E. Thompson; Chongwu Zhou

doi:10.1021/nn900086c

Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes

Fumiaki N. Ishikawa, Hsiao Kang Chang, Marco Curreli, Hsiang I. Liao, C. Anders Olson, Po Chiang Chen, Rui Zhang, Richard W. Roberts, Ren Sun, Richard J. Cote, Mark E. Thompson, Chongwu Zhou

Research output: Contribution to journal › Article › peer-review

162 Scopus citations

Abstract

Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In ₂O ₃ nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.

Original language	English
Pages (from-to)	1219-1224
Number of pages	6
Journal	ACS nano
Volume	3
Issue number	5
DOIs	https://doi.org/10.1021/nn900086c
State	Published - May 26 2009

Keywords

Antibody mimetic protein
Biosensor
Nanowire
Nucleocapsid (N) protein

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10.1021/nn900086c

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title = "Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes",

abstract = "Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In 2O 3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.",

keywords = "Antibody mimetic protein, Biosensor, Nanowire, Nucleocapsid (N) protein",

author = "Ishikawa, {Fumiaki N.} and Chang, {Hsiao Kang} and Marco Curreli and Liao, {Hsiang I.} and Olson, {C. Anders} and Chen, {Po Chiang} and Rui Zhang and Roberts, {Richard W.} and Ren Sun and Cote, {Richard J.} and Thompson, {Mark E.} and Chongwu Zhou",

year = "2009",

month = may,

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doi = "10.1021/nn900086c",

language = "English",

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AU - Ishikawa, Fumiaki N.

AU - Chang, Hsiao Kang

AU - Curreli, Marco

AU - Liao, Hsiang I.

AU - Olson, C. Anders

AU - Chen, Po Chiang

AU - Zhang, Rui

AU - Roberts, Richard W.

AU - Sun, Ren

AU - Cote, Richard J.

AU - Thompson, Mark E.

AU - Zhou, Chongwu

PY - 2009/5/26

Y1 - 2009/5/26

N2 - Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In 2O 3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.

AB - Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In 2O 3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.

KW - Antibody mimetic protein

KW - Biosensor

KW - Nanowire

KW - Nucleocapsid (N) protein

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Label-free, electrical detection of the SARS virus n-protein with nanowire biosensors utilizing antibody mimics as capture probes

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Keywords

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