Abstract
Antibody mimic proteins (AMPs) are polypeptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10 kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In 2O 3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at subnanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors.
Original language | English |
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Pages (from-to) | 1219-1224 |
Number of pages | 6 |
Journal | ACS nano |
Volume | 3 |
Issue number | 5 |
DOIs | |
State | Published - May 26 2009 |
Keywords
- Antibody mimetic protein
- Biosensor
- Nanowire
- Nucleocapsid (N) protein