TY - GEN
T1 - Lab-on-a-card assay for enteric pathogens
AU - Weigl, B. H.
AU - Gerdes, J.
AU - Tarr, P.
AU - Yager, P.
AU - Dillman, L.
AU - Gerlach, J.
AU - Steele, M.
AU - Peck, R.
AU - Ramachandran, S.
AU - Lemba, M.
AU - Hoekstra, D.
AU - Kokoris, M.
AU - Nabavi, M.
AU - Battrell, F.
AU - Denno, D. M.
AU - Klein, E. J.
N1 - Publisher Copyright:
© 2006 TRF.
PY - 2006
Y1 - 2006
N2 - We describe the functional elements of a diagnostic instrument and disposable enteric card (DEC) system under development that rapidly identifies and differentiates Shigella dysenteriae serotype 1, Escherichia coli O157:H7, Campylobacter jejuni, and Salmonella and Shigella species in stool samples. These elements, currently realized as microfluidic subcircuits on individual cards, are (1) whole pathogen immunocapture, (2) nucleic acid extraction, (3) on-chip rapid PCR, and (4) lateral flow detection of amplicons. The system utilizes a low-cost disposable lab-on-a-card platform designed to identify enteric bacterial pathogens in patients with acute diarrhea. Special emphasis is placed on the utility of the device to diagnose both intentionally released enteric biothreat agents as well as to provide a platform to identify infections that are common worldwide. All reagents are stored in dry form on the card. In this paper we report on the design and validation of individual subcircuits, identification and validation of capture antibodies and strategy for organism immunocapture, and identification and validation of specific PCR primer sequences for over 200 clinical isolates of enteric pathogens as well as spiked and pathogenic stool samples.
AB - We describe the functional elements of a diagnostic instrument and disposable enteric card (DEC) system under development that rapidly identifies and differentiates Shigella dysenteriae serotype 1, Escherichia coli O157:H7, Campylobacter jejuni, and Salmonella and Shigella species in stool samples. These elements, currently realized as microfluidic subcircuits on individual cards, are (1) whole pathogen immunocapture, (2) nucleic acid extraction, (3) on-chip rapid PCR, and (4) lateral flow detection of amplicons. The system utilizes a low-cost disposable lab-on-a-card platform designed to identify enteric bacterial pathogens in patients with acute diarrhea. Special emphasis is placed on the utility of the device to diagnose both intentionally released enteric biothreat agents as well as to provide a platform to identify infections that are common worldwide. All reagents are stored in dry form on the card. In this paper we report on the design and validation of individual subcircuits, identification and validation of capture antibodies and strategy for organism immunocapture, and identification and validation of specific PCR primer sequences for over 200 clinical isolates of enteric pathogens as well as spiked and pathogenic stool samples.
UR - http://www.scopus.com/inward/record.url?scp=79958124245&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:79958124245
T3 - Technical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop
SP - 171
EP - 176
BT - 2006 Solid-State Sensors, Actuators, and Microsystems Workshop, Hilton Head 2006
A2 - Kenny, Thomas W.
A2 - Spangler, Leland
PB - Transducer Research Foundation
T2 - 13th Solid-State Sensors, Actuators, and Microsystems Workshop, Hilton Head 2006
Y2 - 4 June 2006 through 8 June 2006
ER -