@inbook{ead8925140d74d07b9b51192ac83b403,
title = "Kinetics of motor protein translocation on single-Stranded DNA",
abstract = "The translocation of nucleic acid motor proteins along DNA or RNA can be studied in ensemble experiments by monitoring either the kinetics of the arrival of the protein at a specific site on the nucleic acid filament (generally one end of the filament) or the kinetics of ATP hydrolysis by the motor protein during translocation. The pre-steady state kinetic data collected in ensemble experiments can be analyzed by simultaneous global non-linear least squares (NLLS) analysis using a simple sequential {"}n-step{"} mechanism to obtain estimates of the rate-limiting step(s) in the translocation cycle, the average {"}kinetic step-size,{"} and the efficiency of coupling ATP binding and hydrolysis to translocation.",
keywords = "ATPase, Translocase, helicase, kinetics, motor protein",
author = "Fischer, {Christopher J.} and Lake Wooten and Tomko, {Eric J.} and Lohman, {Timothy M.}",
year = "2010",
doi = "10.1007/978-1-60327-355-8_4",
language = "English",
isbn = "9781603273541",
series = "Methods in Molecular Biology",
pages = "45--56",
booktitle = "Helicases",
}