Kilohertz two-photon brain imaging in awake mice

Tong Zhang; Oscar Hernandez; Radosław Chrapkiewicz; Adam Shai; Mark J. Wagner; Yanping Zhang; Cheng Hsun Wu; Jin Zhong Li; Masatoshi Inoue; Yiyang Gong; Biafra Ahanonu; Hongkui Zeng; Haruhiko Bito; Mark J. Schnitzer

doi:10.1038/s41592-019-0597-2

Kilohertz two-photon brain imaging in awake mice

Tong Zhang
, Oscar Hernandez
, Radosław Chrapkiewicz
, Adam Shai
, Mark J. Wagner
, Yanping Zhang
, Cheng Hsun Wu
, Jin Zhong Li
, Masatoshi Inoue
, Yiyang Gong
, Biafra Ahanonu
, Hongkui Zeng
, Haruhiko Bito
, Mark J. Schnitzer

Research output: Contribution to journal › Article › peer-review

93 Scopus citations

Abstract

Two-photon microscopy is a mainstay technique for imaging in scattering media and normally provides frame-acquisition rates of ~10–30 Hz. To track high-speed phenomena, we created a two-photon microscope with 400 illumination beams that collectively sample 95,000–211,000 µm² areas at rates up to 1 kHz. Using this microscope, we visualized microcirculatory flow, fast venous constrictions and neuronal Ca²⁺ spiking with millisecond-scale timing resolution in the brains of awake mice.

Original language	English
Pages (from-to)	1119-1122
Number of pages	4
Journal	Nature Methods
Volume	16
Issue number	11
DOIs	https://doi.org/10.1038/s41592-019-0597-2
State	Published - Nov 1 2019

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title = "Kilohertz two-photon brain imaging in awake mice",

abstract = "Two-photon microscopy is a mainstay technique for imaging in scattering media and normally provides frame-acquisition rates of \textasciitilde{}10–30 Hz. To track high-speed phenomena, we created a two-photon microscope with 400 illumination beams that collectively sample 95,000–211,000 µm2 areas at rates up to 1 kHz. Using this microscope, we visualized microcirculatory flow, fast venous constrictions and neuronal Ca2+ spiking with millisecond-scale timing resolution in the brains of awake mice.",

author = "Tong Zhang and Oscar Hernandez and Rados{\l}aw Chrapkiewicz and Adam Shai and Wagner, \{Mark J.\} and Yanping Zhang and Wu, \{Cheng Hsun\} and Li, \{Jin Zhong\} and Masatoshi Inoue and Yiyang Gong and Biafra Ahanonu and Hongkui Zeng and Haruhiko Bito and Schnitzer, \{Mark J.\}",

note = "Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.",

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doi = "10.1038/s41592-019-0597-2",

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T1 - Kilohertz two-photon brain imaging in awake mice

AU - Zhang, Tong

AU - Hernandez, Oscar

AU - Chrapkiewicz, Radosław

AU - Shai, Adam

AU - Wagner, Mark J.

AU - Zhang, Yanping

AU - Wu, Cheng Hsun

AU - Li, Jin Zhong

AU - Inoue, Masatoshi

AU - Gong, Yiyang

AU - Ahanonu, Biafra

AU - Zeng, Hongkui

AU - Bito, Haruhiko

AU - Schnitzer, Mark J.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Two-photon microscopy is a mainstay technique for imaging in scattering media and normally provides frame-acquisition rates of ~10–30 Hz. To track high-speed phenomena, we created a two-photon microscope with 400 illumination beams that collectively sample 95,000–211,000 µm2 areas at rates up to 1 kHz. Using this microscope, we visualized microcirculatory flow, fast venous constrictions and neuronal Ca2+ spiking with millisecond-scale timing resolution in the brains of awake mice.

AB - Two-photon microscopy is a mainstay technique for imaging in scattering media and normally provides frame-acquisition rates of ~10–30 Hz. To track high-speed phenomena, we created a two-photon microscope with 400 illumination beams that collectively sample 95,000–211,000 µm2 areas at rates up to 1 kHz. Using this microscope, we visualized microcirculatory flow, fast venous constrictions and neuronal Ca2+ spiking with millisecond-scale timing resolution in the brains of awake mice.

UR - https://www.scopus.com/pages/publications/85074301998

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DO - 10.1038/s41592-019-0597-2

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SN - 1548-7091

VL - 16

SP - 1119

EP - 1122

JO - Nature Methods

JF - Nature Methods

IS - 11

ER -

Kilohertz two-photon brain imaging in awake mice

Abstract

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