Killing of burkitt-lymphoma-derived daudi cells by ultraviolet-inactivated vaccinia virus

Leora Grunwald-Beard, Haim Gamliel, Gisia Parag, Suresh Vedantham, Zichria Zakay-Rones

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Interaction of active and UV-inactivated vaccinia virus at high multiplicity caused cytological changes and inhibition in cellular protein and DNA synthesis, thus arresting the multiplication of Burkitt-lymphomaderived Daudi cells and eventually killing the cells. Adsorption to the cells but the lack of penetration was evident by immunofluorescence, electron microscopy and [3H]thymidine-labeled virus incorporation. Viral DNA synthesis or virus replication was not demonstrated. Thus, it appears that the massive adsorption of viral particles, active or UV-inactivated, or possibly a "toxic" component that resides in the virion, damages the plasma membrane and may be responsible for killing the cells by a mechanism of lysis from without.

Original languageEnglish
Pages (from-to)561-567
Number of pages7
JournalJournal of Cancer Research and Clinical Oncology
Volume117
Issue number6
DOIs
StatePublished - Nov 1 1991

Keywords

  • Cytotoxicity
  • Daudi-Burkitt lymphoma cells
  • UV-inactivated virus
  • Vaccinia virus

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