Keratin particle-induced osteolysis: A mouse model of iflammatory bone remodeling related to cholesteatoma

Richard A. Chole, Ruth M. Hughes, Brian T. Faddis

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We implanted keratin and poly(methyl methacrylate) (PMMA) particles to the surface of mouse calvariae to produce a quantitative, localized, inflammatory bone remodeling similar to that seen in cholesteatoma. Both types of particles resulted in increased osteoclast density compared with controls. Osteoclasts infiltrated from marrow and vascular spaces and were active at the periphery of these spaces leading to significant bone remodeling, as demonstrated by the incorporation of bone-labelling fluorophores. Osteoclasts were rarely found on the surface of the calvariae, and mineral apposition rate at the ventral surface was not altered in keratin-implanted animals compared with nonoperated controls. While not useful for the study of the root cause of cholesteatoma, this model will allow the study of pathologic bone remodeling related to cholesteatoma in a genetically defined animal.

Original languageEnglish
Pages (from-to)65-71
Number of pages7
JournalJARO - Journal of the Association for Research in Otolaryngology
Volume2
Issue number1
DOIs
StatePublished - 2001

Keywords

  • Bone resorption
  • Cholesteatoma
  • Keratin
  • Osteoclast

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