KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene

Mark D. Rose, Leanne M. Misra, Joseph P. Vogel

Research output: Contribution to journalArticle

503 Scopus citations


The yeast KAR2 gene was isolated by complementation of a mutation that blocks nuclear fusion. The predicted KAR2 protein sequence is most homologous to mammalian BiP/GRP78 and has several structural features in common with it: a functional secretory signal sequence, a yeast endoplasmic reticulum retention signal (HDEL) at the carboxyl terminus, and the absence of potential N-linked glycosylation sites. Moreover KAR2 is regulated like BiP/GRP78: the level of mRNA is increased by drug treatments and mutations that cause accumulation of secretory precursors in the endoplasmic reticulum. However, unlike BiP/GRP78, KAR2 is also regulated by heat shock. Deletion of the KAR2 gene generated a recessive lethal mutation, showing that BiP/GRP78 function is required for cell viability.

Original languageEnglish
Pages (from-to)1211-1221
Number of pages11
Issue number7
StatePublished - Jun 30 1989
Externally publishedYes

Fingerprint Dive into the research topics of 'KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene'. Together they form a unique fingerprint.

  • Cite this