Jasmonate Hypersensitive 3 negatively regulates both jasmonate and ethylene-mediated responses in Arabidopsis

Kwi Mi Chung, Agnes J. Demianski, Gregory A. Harrison, Neva Laurie-Berry, Nobutaka Mitsuda, Barbara N. Kunkel

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Jasmonate (JA) is an important hormone involved in regulating diverse responses to environmental factors as well as growth and development, and its signalling is influenced by other hormones such as ethylene (ET). However, our understanding of the regulatory relationship between the JA and ET signalling pathways is limited. In this study, we isolated an Arabidopsis JA-hypersensitive mutant, jah3 (jasmonate hypersensitive3)-1. Map-based cloning revealed that the JAH3 gene corresponds to At4g16535. JAH3 encodes a protein of unknown function whose amino acid sequence has similarity to leukocyte receptor cluster-like protein. The mutation in jah3-1 is caused by a single nucleotide change from A to T at position 220 of 759 bp. Using CRISPR-Cas9, we generated a second allele, jah3-2, that encodes a truncated protein. Both of these loss-of-function alleles resulted in hypersensitivity to JA, ET-induced root growth inhibition, and accelerated dark-induced senescence. Double mutant analyses employing coronatine insensitive 1 (coi1) and ethylene insensitive 3 (ein3) mutants (jah3 coi1 and jah3 ein3) demonstrated that the hypersensitive phenotypes of the jah3 mutants are mediated by JA and ET signalling components COI1 and EIN3. Therefore, we propose that JAH3 is a negative regulator of both JA and ET signalling.

Original languageEnglish
Pages (from-to)5067-5083
Number of pages17
JournalJournal of Experimental Botany
Volume73
Issue number14
DOIs
StatePublished - Aug 11 2022

Keywords

  • Arabidopsis
  • JA signalling
  • cross-talk
  • dark-induced senescence
  • ethylene signalling
  • negative regulator

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