Isolation/Purification of Proteins

Gregory A. Grant

doi:10.1016/B978-0-12-821618-7.00060-2

Isolation/Purification of Proteins

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Scientists have been engaged in the endeavor of protein isolation since it was first realized that proteins were individual molecular entities that could be differentiated from their environment. It is usually necessary to obtain them in pure form in order to determine their function and define their properties. Differences in the location, solubility, charge, size, and biological affinity of each protein can be exploited to produce a pure protein. This article presents the various methods available to accomplish a successful purification.

Original language	English
Title of host publication	Encyclopedia of Cell Biology
Subtitle of host publication	Volume 1-6, Second Edition
Publisher	Elsevier
Pages	74-85
Number of pages	12
Volume	1
ISBN (Electronic)	9780128216248
DOIs	https://doi.org/10.1016/B978-0-12-821618-7.00060-2
State	Published - Jan 1 2022

Keywords

Adsorption chromatography
Affinity chromatography
Affinity tag
Batch adsorption
Column chromatography
Differential precipitation
Ion-exchange chromatography
Protein
Protein isolation
Protein purification and Size exclusion chromatography

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10.1016/B978-0-12-821618-7.00060-2

Cite this

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title = "Isolation/Purification of Proteins",

abstract = "Scientists have been engaged in the endeavor of protein isolation since it was first realized that proteins were individual molecular entities that could be differentiated from their environment. It is usually necessary to obtain them in pure form in order to determine their function and define their properties. Differences in the location, solubility, charge, size, and biological affinity of each protein can be exploited to produce a pure protein. This article presents the various methods available to accomplish a successful purification.",

keywords = "Adsorption chromatography, Affinity chromatography, Affinity tag, Batch adsorption, Column chromatography, Differential precipitation, Ion-exchange chromatography, Protein, Protein isolation, Protein purification and Size exclusion chromatography",

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AB - Scientists have been engaged in the endeavor of protein isolation since it was first realized that proteins were individual molecular entities that could be differentiated from their environment. It is usually necessary to obtain them in pure form in order to determine their function and define their properties. Differences in the location, solubility, charge, size, and biological affinity of each protein can be exploited to produce a pure protein. This article presents the various methods available to accomplish a successful purification.

KW - Adsorption chromatography

KW - Affinity chromatography

KW - Affinity tag

KW - Batch adsorption

KW - Column chromatography

KW - Differential precipitation

KW - Ion-exchange chromatography

KW - Protein

KW - Protein isolation

KW - Protein purification and Size exclusion chromatography

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U2 - 10.1016/B978-0-12-821618-7.00060-2

DO - 10.1016/B978-0-12-821618-7.00060-2

M3 - Chapter

AN - SCOPUS:85159010405

VL - 1

SP - 74

EP - 85

BT - Encyclopedia of Cell Biology

PB - Elsevier

ER -

Isolation/Purification of Proteins

Abstract

Keywords

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