Isolation/Purification of Proteins

Scientists have been engaged in the endeavor of protein isolation since it was first realized that proteins were individual molecular entities that could be differentiated from their environment. It is usually necessary to obtain them in pure form in order to determine their function and define their properties. Differences in the location, solubility, charge, size, and biological affinity of each protein can be exploited to produce a pure protein. This article presents the various methods available to accomplish a successful purification.