Isolation/Purification of Proteins

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Scientists have been engaged in the endeavor of protein isolation since it was first realized that proteins were individual molecular entities that could be differentiated from their environment. It is usually necessary to obtain them in pure form in order to determine their function and define their properties. Differences in the location, solubility, charge, size, and biological affinity of each protein can be exploited to produce a pure protein. This article presents the various methods available to accomplish a successful purification.

Original languageEnglish
Title of host publicationMolecular Cell Biology
PublisherElsevier Inc.
Pages66-74
Number of pages9
Volume1
ISBN (Electronic)9780123944474
ISBN (Print)9780123947963
DOIs
StatePublished - 2016

Keywords

  • Adsorption chromatography
  • Affinity chromatography
  • Affinity tag
  • Batch adsorption
  • Column chromatography
  • Differential precipitation
  • Ion-exchange chromatography
  • Protein
  • Protein isolation
  • Protein purification
  • Size exclusion chromatography

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