Abstract

Current single-cell RNA sequencing (scRNA-seq) protocols are limited by the number of cells that can be simultaneously sequenced, restricting the ability to resolve heterogeneity of rare cell types. We describe here a protocol for rapid isolation of myeloid cells from tumor-harboring mouse cerebellum without cell sorting to minimize cell damage for scRNA-seq. This protocol includes the procedures for further enrichment of myeloid cells using CD11b+ magnetic beads, followed by the generation of scRNA library and sequencing analysis. For complete details on the use and execution of this protocol, please refer to Dang et al. (2021).

Original languageEnglish
Article number100957
JournalSTAR Protocols
Volume2
Issue number4
DOIs
StatePublished - Dec 17 2021

Keywords

  • Cancer
  • Cell Biology
  • Cell isolation
  • Immunology
  • RNAseq
  • Sequencing
  • Single Cell

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